Document 0075
 DOCN  M9650075
 TI    Deoxyglucose uptake by mouse astrocytes: effects of temperature and
       retrovirus infection.
 DT    9605
 AU    Vann JM; Goldman AJ; Szurek PF; Brooks BR; Neurology Service, William S.
       Middleton Memorial Veterans; Hospital, Madison, WI 53705-2286, USA.
 SO    Neurochem Res. 1995 Sep;20(9):1013-20. Unique Identifier : AIDSLINE
       MED/96117160
 AB    Deoxyglucose uptake by FVB/N mouse astrocytes was studied before and
       after infection by ts1 retrovirus which causes a neurodegenerative
       disease in mice similar to HIV-1 encephalopathy in man. The
       Michaelis-Menten kinetic parameters, Km and Vmax, of 2-deoxy-D-glucose
       uptake by brain and cerebellar astrocytes were measured following
       culture at 34 degrees C where ts1 retrovirus replicates optimally, and
       at 37 degrees C. Compared to astrocytes cultured at 37 degrees C,
       astrocytes cultured at 34 degrees C had increased Km and decreased
       deoxyglucose uptake despite increased or unchanged Vmax. Following ts1
       retrovirus infection, brain astrocyte deoxyglucose uptake doubled [132%]
       associated with decreased Km but unchanged Vmax, whereas cerebellar
       astrocyte deoxyglucose uptake doubled [102%] associated with increased
       Vmax but unchanged Km. These observations of altered deoxyglucose uptake
       kinetic parameters following retrovirus infection indicate different
       neurochemical mechanisms for the regional variation in deoxyglucose
       uptake observed following retrovirus infection of the CNS in vivo.
 DE    Animal  Astrocytes/*METABOLISM  AIDS Dementia Complex/*METABOLISM
       Cells, Cultured  Comparative Study  Deoxyglucose/*METABOLISM
       Fibroblasts/METABOLISM  *Heat  Human  Kinetics  Mice  Retroviridae
       Infections/*METABOLISM  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       Non-P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).