Document 0089
 DOCN  M9650089
 TI    TCR-independent induction of low responsiveness by chemically fixed
       cells in alloreactive CTL clones and its prevention through cognate
       cell-cell interaction.
 DT    9605
 AU    Lwin T; Nakashima I; Nagase F; Department of Immunology, Nagoya
       University School of Medicine,; Aichi, Japan.
 SO    Microbiol Immunol. 1995;39(7):509-15. Unique Identifier : AIDSLINE
       MED/96025421
 AB    We established BALB/c-derived CD8+ CTL clones D2-22 (V beta 6+), D2-23
       (V beta 8+) and D2-24 (V beta 8+) specific for B10.D2 minor H antigen.
       D2-22 and D2-23 proliferated without producing IL-2 in response to
       X-ray-irradiated antigenic cells, Con A, aCD3, PMA and IL-2.
       Paraformaldehyde-fixed antigenic spleen cells neither induced
       proliferation in the presence of costimulatory cells nor inhibited
       responses to irradiated antigenic cells added simultaneously. Unlike the
       previously reported results with IL-2-producing CTL clones and Th1
       clones, the fixed antigenic cells failed to induce antigen-specific
       unresponsiveness in these IL-2-non-producing CTL clones. Instead, the
       responsiveness of these clones to fresh stimulation was found to be
       reduced severely after 2 days in the culture added with either antigenic
       or syngeneic fixed cells. Induction of their antigen-nonspecific low
       responsiveness by the fixed cells was prevented by adding irradiated
       syngeneic cells into the culture or even by increasing the concentration
       of responder D2-23 cells. Close contact of D2-23 and irradiated
       syngeneic cells was required to prevent the reduction of the
       responsiveness, although this cognate cell-cell interaction could be
       replaced by exogenously added IL-2 or PMA. Cytolytic and tumor cell
       growth inhibitory activities of D2-23 were also reduced by incubation
       with the fixed cells, which was prevented by the addition of irradiated
       syngeneic cells. These findings showed the unique properties of
       IL-2-nonproducing CTL clones in signal requirements for maintaining
       normal responsiveness for proliferation and cytolytic activity.
 DE    Animal  Antigen-Presenting Cells/IMMUNOLOGY  *Cell Communication/DRUG
       EFFECTS/RADIATION EFFECTS  Clone Cells  Cytotoxicity, Immunologic
       CD8-Positive T-Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY/RADIATION  EFFECTS
       Fixatives  Formaldehyde/PHARMACOLOGY  Interleukin-2/BIOSYNTHESIS
       Isoantigens/*IMMUNOLOGY  Lymphocyte Transformation/IMMUNOLOGY  Mice
       Mice, Inbred BALB C  Polymers/PHARMACOLOGY  Receptors, Antigen,
       T-Cell/*IMMUNOLOGY  Spleen/CYTOLOGY  T-Lymphocytes, Cytotoxic/DRUG
       EFFECTS/*IMMUNOLOGY/RADIATION  EFFECTS  JOURNAL ARTICLE

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