Document 0090
 DOCN  M9650090
 TI    Sequential involvement of NK cells and CD8+ T cells in granuloma
       formation of Rhodococcus aurantiacus-infected mice.
 DT    9605
 AU    Asano M; Nakane A; Kohanawa M; Minagawa T; Department of Microbiology,
       Hokkaido University School of; Medicine, Japan.
 SO    Microbiol Immunol. 1995;39(7):499-507. Unique Identifier : AIDSLINE
       MED/96025420
 AB    We investigated the effect of in vivo administration of antibodies
       against T-cell subsets and natural killer (NK) cells on endogenous gamma
       interferon (IFN-gamma) production and granuloma formation in Rhodococcus
       aurantiacus-infected mice. High titers of endogenous IFN-gamma were
       detected in the extracts of the livers and spleens during 24 hr of the
       infection, reaching the peak at 8 hr, and the IFN-gamma production was
       reduced by in vivo administration of anti-NK 1.1 monoclonal antibody
       (MAb) or antibody against asialo GM1+ cells. Endogenous IFN-gamma
       declined until 2 days of the infection, then reappeared from 1 week and
       peaked at 3 weeks. Endogenous IFN-gamma at 1 and 3 weeks was reduced by
       in vivo administration of anti-CD8 MAb, but not by anti-CD4 MAb or
       anti-NK 1.1 MAb. Granulomatous lesions in the livers and spleens began
       to appear from 1 week of the infection and developed in 3 weeks. In vivo
       administration of rat anti-IFN-gamma MAb reduced the development of
       granulomas. In addition, granuloma formation was reduced by depletion of
       NK cells prior to the infection or depletion of CD8+ T cells at 1 week
       of the infection. Based on these findings, it is presumed that the
       biphasic production of IFN-gamma is attributable to NK cells in the
       early phase of the infection and CD8+ T cells in the phase of granuloma
       formation, and that granuloma formation is regulated by NK cells and
       CD8+ T cells through the secretion of endogenous IFN-gamma.
 DE    Actinomycetales Infections/*COMPLICATIONS  Animal  Antibodies,
       Monoclonal/PHARMACOLOGY  Colony Count, Microbial  CD4-Positive
       T-Lymphocytes/PHYSIOLOGY  CD8-Positive T-Lymphocytes/*PHYSIOLOGY  Female
       Granuloma/*ETIOLOGY/PATHOLOGY  Interferon Type
       II/BIOSYNTHESIS/IMMUNOLOGY  Killer Cells, Natural/*PHYSIOLOGY
       Liver/METABOLISM/MICROBIOLOGY  Liver Diseases/*ETIOLOGY/PATHOLOGY
       Lymphocyte Depletion  Mice  Mice, Inbred C57BL  *Rhodococcus
       Spleen/METABOLISM/MICROBIOLOGY  Splenic Diseases/*ETIOLOGY/PATHOLOGY
       JOURNAL ARTICLE

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