Document 0091
 DOCN  M9650091
 TI    Overproduction, purification, and diagnostic use of the recombinant
       HIV-1 Gag proteins, the precursor protein p55 and the processed products
       p17, p24, and p15.
 DT    9605
 AU    Saito A; Morimoto M; Ohara T; Takamizawa A; Nakata A; Shinagawa H;
       Department of Molecular Microbiology, Osaka University, Japan.
 SO    Microbiol Immunol. 1995;39(7):473-83. Unique Identifier : AIDSLINE
       MED/96025417
 AB    HIV-1 Gag protein precursor p55, and its processed products, p17, p24,
       and p15 were overproduced in Escherichia coli and purified to near
       homogeneity. To study the antigenic properties and the potentiality as
       the diagnostic and prognostic reagents, varying amounts of the purified
       Gag proteins were dotted onto the polyvinylidene difluoride membrane and
       reacted with 40 sera of HIV-1-infected individuals (35 AC, 1 ARC, and 4
       AIDS patients) and 10 sera of normal healthy donors. p55 reacted with 40
       (100%) sera of HIV-1 carriers, while p17, p24, and p15 reacted with 37
       (92.5%), 35 (87.5%) and 34 (85%) of the 40 sera of HIV-1 carriers,
       respectively. On the whole, the reaction of p55 was especially strong
       and that of p15 was the weakest. p55 showed the strongest reaction among
       the four Gag proteins with all specimens, and it showed a positive
       reaction with a carrier serum with which none of the processed Gag
       proteins showed a positive reaction. Therefore, p55 is the most useful
       antigen among the four Gag proteins for detection of the Gag antibodies
       and may even be one of the most useful antigens for the diagnosis of
       HIV-1 infection.
 DE    Chromatography, Gel  Electrophoresis, Polyacrylamide Gel  Escherichia
       coli/GENETICS/METABOLISM  Gene Expression  Gene Products,
       gag/BIOSYNTHESIS/*DIAGNOSTIC USE/ISOLATION & PURIF  Human  HIV
       Antibodies/ANALYSIS  HIV Antigens/BIOSYNTHESIS/*DIAGNOSTIC USE/ISOLATION
       & PURIF  HIV Core Protein p24/BIOSYNTHESIS/*DIAGNOSTIC USE/ISOLATION &
       PURIF  HIV Infections/*DIAGNOSIS  HIV-1/GENETICS/*METABOLISM
       Immunoblotting  Plasmids  Protein Precursors/BIOSYNTHESIS/*DIAGNOSTIC
       USE/ISOLATION & PURIF  Recombinant Proteins  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).