Document 0101
 DOCN  M9650101
 TI    Calbindin D28K-containing neurons, and not HSP70-expressing neurons, are
       more resistant to HIV-1 envelope (gp120) toxicity in cortical cell
       cultures.
 DT    9605
 AU    Diop AG; Lesort M; Esclaire F; Dumas M; Hugon J; Cellular Neurobiology
       Unit-Laboratory of Histology, Medical; Institute of Cellular and
       Molecular Biology, Faculty of Medicine,; Limoges, France.
 SO    J Neurosci Res. 1995 Oct 1;42(2):252-8. Unique Identifier : AIDSLINE
       MED/96122605
 AB    HIV-1-associated cognitive/motor complex is one of the major
       neurological complications of AIDS and is associated with neuronal loss.
       Gp120, the HIV envelope protein, is toxic for neurons in cultures and
       produces a rise in intracytosolic calcium. This neurotoxicity is
       dose-dependent and time-dependent. We evaluated the selective gp120
       toxicity in primary neuronal cultures for calbindin-free and
       calbindin-containing neurons with semi-quantitative immunocytochemistry
       using an anti-calbindin D28K monoclonal antibody. The number of
       immunolabelled neurons was inversely correlated to neuronal survival. In
       cultures exposed to gp120 (100 pM) for 24 hr the neuronal survival of
       initial platings was 19.7 +/- 2.1% and the percentage of neuronal
       survival was 84.6 +/- 4.9% in control cultures exposed to the vehicle.
       The corresponding percentages of immunolabelled neurons were 85.0 +/-
       2.1% in treated cultures and 23.6 +/- 3.1% in control cultures (P <
       0.001). The expression of heat shock proteins by heating cell cultures
       did not protect neurons from gp120 toxicity. These results suggest that
       calbindin D2K28-containing neurons are more resistant to gp120-toxicity
       in this cell culture system.
 DE    Animal  Biological Transport  Calcium/METABOLISM  Calcium-Binding
       Protein, Vitamin D-Dependent/*ANALYSIS/PHYSIOLOGY  Cell Survival  Cells,
       Cultured  Cerebral Cortex/*CYTOLOGY/EMBRYOLOGY  Comparative Study
       Dose-Response Relationship, Drug  Heat  Heat-Shock Proteins
       70/*BIOSYNTHESIS/GENETICS  HIV Envelope Protein gp120/GENETICS/*TOXICITY
       HIV-1/GENETICS  Immunoenzyme Techniques  Macrophages/CYTOLOGY  Nerve
       Tissue Proteins/*ANALYSIS/BIOSYNTHESIS/GENETICS/PHYSIOLOGY
       Neurons/CHEMISTRY/*DRUG EFFECTS  Rats  Recombinant Proteins/TOXICITY
       Support, Non-U.S. Gov't  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).