Document 0119
 DOCN  M9650119
 TI    Primary and secondary syphilis lesions contain mRNA for Th1 cytokines.
 DT    9605
 AU    Van Voorhis WC; Barrett LK; Koelle DM; Nasio JM; Plummer FA; Lukehart
       SA; Department of Medicine, University of Washington, Seattle;
       98195-7185, USA.
 SO    J Infect Dis. 1996 Feb;173(2):491-5. Unique Identifier : AIDSLINE
       GENBANK
 AB    Phagocytosis of Treponema pallidum by cytokine-activated macrophages
       aids bacterial clearance and lesion resolution in early syphilis. To
       investigate the cytokine profiles of cells infiltrating primary and
       secondary syphilis lesions, reverse transcription and polymerase chain
       reaction (RT-PCR) were used to detect cytokine mRNA in 13 lesion
       biopsies. Both primary and secondary lesions contained mRNA encoding
       interleukin (IL)-2, interferon-gamma (IFN-gamma), IL-12p40, and IL-10.
       In contrast to a lesion from a patient with recurrent herpes simplex
       virus type 2, no message for IL-4 could be detected in any of the
       syphilis lesions, and 10 of 13 had no mRNA for IL-5 or IL-13. These
       findings are consistent with a Th1-predominant local cellular response
       activating macrophages and support the hypothesis that
       IFN-gamma-activated macrophages are primary effectors in treponeme
       clearance.
 DE    Base Sequence  Chancre/*IMMUNOLOGY  Condylomata Acuminata/*IMMUNOLOGY
       Cytokines/*ANALYSIS/GENETICS  DNA Primers/CHEMISTRY  Electrophoresis,
       Agar Gel  Herpes Genitalis/IMMUNOLOGY  Herpesvirus 2, Human/IMMUNOLOGY
       Human  Macrophages/IMMUNOLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction  RNA, Messenger/*ANALYSIS  Support, Non-U.S. Gov't  Support,
       U.S. Gov't, P.H.S.  Th1 Cells/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).