Document 0125
 DOCN  M9650125
 TI    Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during
       experimental human infection.
 DT    9605
 AU    Spinola SM; Orazi A; Arno JN; Fortney K; Kotylo P; Chen CY; Campagnari
       AA; Hood AF; Department of Medicine, Indiana University, Indianapolis,
       USA.
 SO    J Infect Dis. 1996 Feb;173(2):394-402. Unique Identifier : AIDSLINE
       MED/96162098
 AB    Human subjects were experimentally infected with Haemophilus ducreyi for
       up to 2 weeks. Bacterial suspensions were delivered into the epidermis
       and dermis through puncture wounds made by an allergy-testing device.
       Subjects developed papular lesions that evolved into pustules resembling
       natural disease. Some papular lesions resolved spontaneously, indicating
       that host responses may clear infection. Bacteria were shed
       intermittently from lesions, suggesting that H. ducreyi may be
       transmissible before ulceration. Host responses to infection consisted
       primarily of cutaneous infiltrate of polymorphonuclear leukocytes,
       Langerhans cells, macrophages, and CD4 T cells of alpha beta lineage.
       Expression of HLA-DR by keratinocytes was associated with the presence
       of interferon-gamma mRNA in the skin. There was little evidence for
       humoral or peripheral blood mononuclear cell responses to bacterial
       antigens. The cutaneous infiltrate of CD4 cells and macrophages provides
       a mechanism that facilitates transmission of human immunodeficiency
       virus by H. ducreyi.
 DE    Adult  Chancroid/*IMMUNOLOGY/MICROBIOLOGY/PATHOLOGY  Chemotaxis,
       Leukocyte/*IMMUNOLOGY  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       Enzyme-Linked Immunosorbent Assay  Female  Haemophilus
       ducreyi/*IMMUNOLOGY/ISOLATION & PURIF  Human  HLA-DR Antigens/METABOLISM
       Interferon Type II/METABOLISM  Keratinocytes/METABOLISM  Langerhans
       Cells/IMMUNOLOGY  Lymphocyte Subsets  Lymphocyte Transformation
       Macrophages/IMMUNOLOGY  Male  Middle Age  Neutrophils/IMMUNOLOGY
       Polymerase Chain Reaction  RNA, Messenger/METABOLISM
       Skin/*IMMUNOLOGY/MICROBIOLOGY/PATHOLOGY  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).