Document 0132
 DOCN  M9650132
 TI    Establishment and characterization of a murine CD4+ T cell line and
       clone that induce experimental autoimmune uveoretinitis in B10.A mice.
 DT    9605
 AU    Rizzo LV; Silver P; Wiggert B; Hakim F; Gazzinelli RT; Chan CC; Caspi
       RR; Laboratory of Immunology, NEI/NIH, Bethesda, MD 20892-1858, USA.
 SO    J Immunol. 1996 Feb 15;156(4):1654-60. Unique Identifier : AIDSLINE
       MED/96164598
 AB    B10.A mice develop experimental autoimmune uveoretinitis after active
       immunization with the interphotoreceptor retinoid-binding protein
       (IRBP). CD4+ T cells play an important role in the development of the
       disease. In this study we have isolated and characterized a CD4+ T cell
       line and a T cell clone that induce experimental autoimmune
       uveoretinitis when transferred into naive B10.A mice. The cell line was
       isolated from draining lymph nodes of IRBP-immunized animals by repeated
       cycles of IRBP stimulation. The line was shown to be pathogenic after 4
       rounds of in vitro stimulation with IRBP at 5 x 10(6) cells/mouse. A T
       cell clone derived from this line by limiting dilution was shown to be
       pathogenic when the same number of cells was injected; incidence and
       severity of disease, however, were much lower. After 16 rounds of
       IRBP-specific stimulation the cell line was pathogenic at 10(5)
       cells/mouse. Analysis of the V beta repertoire revealed that at this
       point the line was mostly composed of V beta 8.2- and V beta 6-positive
       cells (> 80% of the population). The uveitogenic clone expressed V beta
       8.2. Both the T cell line and the clone elaborated an unrestricted
       lymphokine profile in vitro. However, when these cells were adoptively
       transferred into naive recipients, mRNA isolated from the uveitic retina
       showed only Th1 type cytokines. These data help to characterize the
       nature of pathogenic cells involved in ocular autoimmunity.
 DE    Animal  Cell Line  Cytokines/GENETICS  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  Gene Expression  Gene Rearrangement,
       beta-Chain T-Cell Antigen Receptor  Mice  Receptors, Antigen, T-Cell,
       alpha-beta/GENETICS  Retinitis/*IMMUNOLOGY/PATHOLOGY  Retinol-Binding
       Proteins/IMMUNOLOGY  RNA, Messenger/GENETICS  Th1 Cells/*IMMUNOLOGY
       Uveitis/*IMMUNOLOGY/PATHOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).