Document 0134
 DOCN  M9650134
 TI    IL-12 expression in AIDS-related lymphoma B cell lines.
 DT    9605
 AU    Benjamin D; Sharma V; Kubin M; Klein JL; Sartori A; Holliday J;
       Trinchieri G; Division of Hematology and Oncology, Arthur G. James
       Cancer; Hospital and Research Institute, Columbus, OH 43210, USA.
 SO    J Immunol. 1996 Feb 15;156(4):1626-37. Unique Identifier : AIDSLINE
       MED/96164595
 AB    IL-12 is a 70-kDa heterodimer formed by the 40-kDa heavy chain (p40) and
       the 35-kDa light chain (p35). Twenty-five Burkitt's lymphoma cell lines
       (CL) and seven normal lymphoblastoid B CL were studied. The Burkitt's CL
       included AIDS-associated B CL (AABCL) (7 EBV+/2 EBV-) and non-AABCL (8
       EBV+/8 EBV-). Reverse transcription-PCR detected p40 in EBV+ AABCL (7 of
       7), EBV+ non-AABCL (3 of 8), and normal lymphoblastoid B CL (6 of 6) but
       not in EBV- CL (0 of 10). p35 mRNA was detected in 30 of 30 CL.
       Constitutive secretion of p40 was found in 7 of 7 EBV+ AABCL (range,
       341-18,086 pg/ml) and p70 in 3 of 7 EBV+ AABCL (range, 25-197 pg/ml),
       but in only 1 of 8 EBV+ non-AABCL and 0 of 7 normal lymphoblastoid CL.
       PMA stimulated p40 secretion in 7 of 7 EBV+ AABCL and p70 secretion in 5
       of 7 EBV+ AABCL. PMA also triggered p40 and p70 secretion in 2 EBV+
       non-AABCL and in 3 of 7 normal lymphoblastoid CL. No IL-12 secretion was
       detected in 10 EBV- CL, including EBV- AABCL. The CL produced IL-10, a
       known inhibitor of IL-12, but anti-IL-10 Abs did not neutralize IL-12.
       Similarly, neutralizing anti-IFN gamma Abs or IFN gamma did not affect B
       cell IL-12. For IL-12R studies, reverse transcription-PCR and 125I-IL-12
       binding assays were performed. Although all CL tested showed mRNA
       accumulation for one of the IL-12R components, IL-12 binding sites were
       detected in only 1 of 30 CL. Our data suggest that: 1) AABCL
       constitutively secrete large amounts of IL-12, contrasting with low
       IL-12 production by HIV-1 infected PBMC; 2) lack of IL-12 expression in
       EBV- AABCL suggests that in vivo exposure of B cells to HIV-1 only does
       not induce IL-12 secretion and that both HIV-1 and EBV are required; 3)
       the autocrine-negative effect of IL-10 on IL-12 in monocytes and the
       enhancing effect of IFN gamma on IL-12 secretion do not apply to B cells
       derived from AIDS patients.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*IMMUNOLOGY  Base
       Sequence  Burkitt's Lymphoma/METABOLISM  Cloning, Molecular  DNA
       Primers/CHEMISTRY  Gene Expression  Human  Interferon Type II/METABOLISM
       Interleukin-10/METABOLISM  Interleukin-12/GENETICS/*METABOLISM
       Lymphoma, B-Cell/COMPLICATIONS/*METABOLISM  Molecular Sequence Data
       Receptors, Interleukin/GENETICS/METABOLISM  RNA, Messenger/GENETICS
       Support, U.S. Gov't, P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).