Document 0138
 DOCN  M9650138
 TI    The T cell receptor gene usage by simian immunodeficiency virus
       gag-specific cytotoxic T lymphocytes in rhesus monkeys.
 DT    9605
 AU    Chen ZW; Shen L; Regan JD; Kou Z; Ghim SH; Letvin NL; Harvard Medical
       School, Beth Israel Hospital, Boston, MA 02215,; USA.
       Zchen@Bih.Harvard.edu
 SO    J Immunol. 1996 Feb 15;156(4):1469-75. Unique Identifier : AIDSLINE
       MED/96164575
 AB    MHC class I-restricted CTL play an important role in limiting the spread
       of HIV-1 in the infected individual. Elucidating the molecular
       interactions of CTL with the virus is, therefore, of central importance
       for characterizing the immune control of this infection. In exploring
       this CTL response, we have defined the TCR usage by SIVmac Gag-specific
       CTL in rhesus monkeys. Thirty-nine CTL clones were generated from PBL of
       three SIVmac-infected monkeys expressing the MHC class I Mamu-A*01 gene
       product, all of which were shown to recognize a single SIVmac Gag
       peptide in association with Mamu-A*01. Sixty-six percent of CTL clones
       derived from two monkeys early after infection expressed TCR genes of
       the V beta 13 family; 70% of these V beta 13+ CTL clones expressed a TCR
       heterodimer composed of V alpha 1 and V beta 13 gene products. In
       addition, there appeared to be a selection of a single conserved amino
       acid and restricted CDR3 lengths in junctional regions of TCR
       beta-chains expressed by the V beta 13+ CTL clones. These findings
       indicate significant structural constraints on the CTL-TCR interaction
       with the AIDS virus. Interestingly, 55% of the CTL clones derived from
       the third animal at a later time following infection employed genes of
       the V beta 6 family in their TCR. Despite the preferential use of TCR V
       family genes by the CTL clones, the SIVmac Gag-specific CTL response was
       clearly polyclonal; TCR expressed by these CTL clones displayed varied
       sequences in their CDR3 regions. Other V gene families, including V beta
       23, V alpha 8, and V alpha 20, were used in TCR expressed by SIVmac
       Gag-specific CTL clones. These studies, therefore, indicate that the TCR
       repertoire of SIVmac Gag-specific CTL that share a peptide and MHC class
       I recognition specificity can be diverse. Such a broad CTL-TCR
       repertoire may be advantageous for the host in containing an AIDS virus
       infection.
 DE    Amino Acid Sequence  Animal  Antigens, Viral/CHEMISTRY/IMMUNOLOGY  Base
       Sequence  Clone Cells  Comparative Study  Epitopes  Gene Products,
       gag/IMMUNOLOGY  Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
       Gene Rearrangement, beta-Chain T-Cell Antigen Receptor  Macaca mulatta
       Molecular Sequence Data  Peptides/IMMUNOLOGY  Receptors, Antigen,
       T-Cell, alpha-beta/*GENETICS  Sequence Alignment  Sequence Homology,
       Amino Acid  Support, U.S. Gov't, P.H.S.  SIV/*IMMUNOLOGY  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).