Document 0152
 DOCN  M9650152
 TI    Purification and characterization of a naturally processed hepatitis B
       virus peptide recognized by CD8+ cytotoxic T lymphocytes.
 DT    9605
 AU    Tsai SL; Chen MH; Yeh CT; Chu CM; Lin AN; Chiou FH; Chang TH; Liaw YF;
       Liver Research Unit, Chang Gung Memorial Hospital, Chang Gung; Medical
       College, Taipei, Taiwan.
 SO    J Clin Invest. 1996 Jan 15;97(2):577-84. Unique Identifier : AIDSLINE
       MED/96149188
 AB    In vitro studies in patients with hepatitis B virus (HBV) infection have
       suggested that hepatocytolysis induced by CD8+ cytotoxic T lymphocytes
       (CTLs) is the most important effector pathway in eliminating infected
       cells. The recognition is implicated in the endogenously processed HBV
       antigens in the context of HLA class I molecules presented on the liver
       cell membrane. However, the naturally occurring HBV peptide antigens
       have not yet been demonstrated. We report here that a naturally
       processed peptide antigen P2 was isolated from HLA class I molecules of
       HBV-infected liver cell membrane. The P2 peptide exhibited the activity
       of sensitizing target cells for lysis by CD8+ CTLs. The P2 sequence
       (YVNVNMGLK) purified from liver tissue was in concordance with that
       encoded by the viral genome for the HBV nucleocapsid antigen or HBcAg
       88-96. P2 peptide could also be isolated from the EBV-transformed B
       cells that were transfected by HBcAg-expressing vector. The P2 epitope,
       sharing the HLA-A11 binding motifs, was recognized by HLA-A11-restricted
       CD8+ CTLs. The data provided direct evidence that, in hepatitis B
       patients, antigenic peptides of HBV were processed by hepatocytes,
       presented with the class I MHC molecules, and recognized by CD8+ CTLs.
 DE    Adult  Amino Acid Sequence  Base Sequence  Carcinoma,
       Hepatocellular/IMMUNOLOGY/MICROBIOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Epitopes  Hepatitis B Antigens/*IMMUNOLOGY
       Hepatitis B Core Antigens/*IMMUNOLOGY  Hepatitis B Virus/*IMMUNOLOGY
       Human  HLA-A Antigens/IMMUNOLOGY  Male  Molecular Sequence Data
       Peptides/IMMUNOLOGY  Protein Processing, Post-Translational  Support,
       Non-U.S. Gov't  T-Lymphocyte Subsets/IMMUNOLOGY  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).