Document 0154
 DOCN  M9650154
 TI    Recombinant interferon-alpha selectively inhibits the production of
       interleukin-5 by human CD4+ T cells.
 DT    9605
 AU    Schandene L; Del Prete GF; Cogan E; Stordeur P; Crusiaux A; Kennes B;
       Romagnani S; Goldman M; Department of Immunology, Hopital
       Erasme-Cliniques; Universitaires de Bruxelles, Belgium.
 SO    J Clin Invest. 1996 Jan 15;97(2):309-15. Unique Identifier : AIDSLINE
       MED/96149155
 AB    The effects of recombinant IFN-alpha on the production of IL-5 by human
       CD4+ T cells were first analyzed on resting CD4+ T cells purified from
       normal PBMC and stimulated either with a combination of PMA and
       anti-CD28 mAb or anti-CD3 mAb cross-linked on B7-1/CD32-transfected
       mouse fibroblasts. We found that IFN-alpha profoundly inhibited in a
       dose-dependent manner IL-5 production by resting CD4+ T cells whereas
       IL-10 was upregulated in both systems. The addition of a neutralizing
       anti-IL-10 mAb to PMA and anti-CD28 mAb upregulated IL-5 production by
       resting CD4+ T cells but did not prevent IFN-alpha-induced IL-5
       inhibition. We then analyzed the effect of IFN-alpha on the production
       of cytokines by differentiated type 2 helper (Th2) CD4+CD3- cells
       isolated from peripheral blood of two patients with the
       hypereosinophilic syndrome. In both cases, IFN-alpha markedly inhibited
       IL-5 production while it induced mild upregulation of IL-4 and IL-10.
       Finally, the inhibitory effect of IFN-alpha on IL-5 production was
       confirmed on a panel of Th2 and Th0 clones generated in vitro. In 2 out
       of 6 clones, IL-5 inhibition was associated with upregulation of IL-4
       and IL-10. We conclude that IFN-alpha selectively downregulates IL-5
       synthesis by human CD4+ T cells.
 DE    Animal  Antigens, CD28/PHYSIOLOGY  Base Sequence  CD4-Positive
       T-Lymphocytes/*METABOLISM  DNA Primers/CHEMISTRY  Gene Expression  Human
       Hypereosinophilic Syndrome/IMMUNOLOGY  Interferon Alfa-2b/*PHARMACOLOGY
       Interleukin-10/BIOSYNTHESIS  Interleukin-4/BIOSYNTHESIS
       Interleukin-5/*BIOSYNTHESIS  Lymphocyte Transformation  Mice  Molecular
       Sequence Data  Platelet Glycoprotein GPIIb-IIIa Complex/ANALYSIS  RNA,
       Messenger/GENETICS  Support, Non-U.S. Gov't  Th2 Cells/*METABOLISM
       Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).