Document 0155
 DOCN  M9650155
 TI    Nonisotopic hybridization assay for determination of relative amounts of
       genotypic human immunodeficiency virus type 1 zidovudine resistance.
 DT    9605
 AU    Eastman PS; Boyer E; Mole L; Kolberg J; Urdea M; Holodniy M; Chiron
       Corporation, Emeryville, California 94608, USA.
 SO    J Clin Microbiol. 1995 Oct;33(10):2777-80. Unique Identifier : AIDSLINE
       MED/96087230
 AB    A nonisotopic hybridization assay for human immunodeficiency virus
       genotypic zidovudine resistance determination is described. Biotinylated
       PCR product was hybridized with enzyme-labeled probes for wild-type or
       resistant mutant sequences and detected colorimetrically or
       chemiluminescently in a microplate format. Changes in
       mutant-to-wild-type ratios allow for the monitoring of longitudinal
       patient samples.
 DE    Antiviral Agents/*PHARMACOLOGY  Chemiluminescence  Drug Resistance,
       Microbial/GENETICS  Genotype  Human  HIV Infections/BLOOD  HIV-1/*DRUG
       EFFECTS/GENETICS  Mutation  Nucleic Acid Hybridization  Polymerase Chain
       Reaction  Reverse Transcriptase Inhibitors/*PHARMACOLOGY  RNA-Directed
       DNA Polymerase/*GENETICS  Sensitivity and Specificity  Support, Non-U.S.
       Gov't  Zidovudine/*PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).