Document 0158
 DOCN  M9650158
 TI    Torulopsis glabrata: azole susceptibilities by microdilution
       colorimetric and macrodilution broth assays.
 DT    9605
 AU    Tiballi RN; Zarins LT; He X; Kauffman CA; Department of Internal
       Medicine, Veterans Affairs Medical Center,; Ann Arbor, Michigan 48105,
       USA.
 SO    J Clin Microbiol. 1995 Oct;33(10):2612-5. Unique Identifier : AIDSLINE
       MED/96087196
 AB    Fluconazole and itraconazole MICs were determined by both the standard
       macrodilution method of the National Committee for Clinical Laboratory
       Standards and a colorimetric broth microdilution method for 140 isolates
       of Torulopsis (Candida) glabrata obtained over a 15-year period. Using
       the method of the National Committee for Clinical Laboratory Standards
       the MICs at which 90% of isolates are inhibited (MIC50) for all isolates
       were 32 and 1.6 micrograms/ml for fluconazole and itraconazole,
       respectively. For fluconazole, the MIC90 rose from 16 to > 64
       micrograms/ml when the MIC90s for isolates collected from July 1980 to
       June 1991 were compared with those for isolates collected from July 1991
       to March 1995. For itraconazole, the MIC90s for isolates from the same
       time periods were 0.8 and 3.2 micrograms/ml, respectively. Although for
       isolates from some non-human immunodeficiency virus-infected patients
       the MICs rose, most of the high MICs were found for isolates from human
       immunodeficiency virus-infected patients who had been extensively
       treated with azole drugs for thrush. The colorimetric method yielded
       endpoints that were more definitive; concordances within 2 dilutions for
       the two methods were 87% for fluconazole and 86% for itraconazole.
 DE    Antifungal Agents/*PHARMACOLOGY  Candida/*DRUG EFFECTS
       Candidiasis/COMPLICATIONS/EPIDEMIOLOGY/*MICROBIOLOGY
       Colorimetry/METHODS  Comparative Study  Dose-Response Relationship, Drug
       Fluconazole/*PHARMACOLOGY  Human  HIV
       Infections/COMPLICATIONS/MICROBIOLOGY  Itraconazole/*PHARMACOLOGY
       Microbial Sensitivity Tests/METHODS/STANDARDS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).