Document 0159
 DOCN  M9650159
 TI    Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA
       present in leukocytes.
 DT    9605
 AU    Zipeto D; Morris S; Hong C; Dowling A; Wolitz R; Merigan TC; Rasmussen
       L; Division of Infectious Diseases, Stanford University School of;
       Medicine, California 94305, USA.
 SO    J Clin Microbiol. 1995 Oct;33(10):2607-11. Unique Identifier : AIDSLINE
       MED/96087195
 AB    A quantitative DNA amplification assay for human cytomegalovirus (CMV)
       DNA has been used to evaluate the relationship between quantities of CMV
       DNA in plasma and those in infected leukocytes (WBC) from human
       immunodeficiency virus-infected patients. The target sequence for DNA
       amplification was a region of the immediate-early 1 gene of CMV. The
       quantitation assay uses an internal control that is coamplified with
       each patient sample DNA and contains a sequence for detection by
       colorimetric hybridization with the same bases, but in different order
       than in the CMV immediate-early 1 region used for hybridization of
       amplified patient sample DNA. Results showed that patients with CMV
       disease had more CMV DNA in both WBC and plasma than those without
       disease. However, in this study, copy numbers of CMV DNA in WBC were
       higher than those in plasma. The gB and gH variants were the same in
       plasma and WBC.
 DE    Base Sequence  Comparative Study  Cytomegalovirus
       Infections/*BLOOD/ETIOLOGY/PATHOLOGY  DNA, Viral/BLOOD/*ISOLATION &
       PURIF  Genotype  Human  HIV Infections/*COMPLICATIONS
       Leukocytes/*VIROLOGY  Molecular Sequence Data  Polymerase Chain Reaction
       Semen/CYTOLOGY/VIROLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).