Document 0160
 DOCN  M9650160
 TI    Increased expression of Candida albicans secretory proteinase, a
       putative virulence factor, in isolates from human immunodeficiency
       virus-positive patients.
 DT    9605
 AU    Ollert MW; Wende C; Gorlich M; McMullan-Vogel CG; Borg-von Zepelin M;
       Vogel CW; Korting HC; Department of Dermatology,
       Ludwig-Maximilians-Universitat; Munchen, Germany.
 SO    J Clin Microbiol. 1995 Oct;33(10):2543-9. Unique Identifier : AIDSLINE
       MED/96087184
 AB    The increased prevalence and the severity of oropharyngeal candidiasis
       in human immunodeficiency virus (HIV)-positive patients are attributed
       exclusively to the virus-induced immune deficiency of the host. The
       present study was aimed at answering the question of whether Candida
       albicans secretory proteinase, a putative virulence factor of the
       opportunistic C. albicans yeast, has any potential influence on the
       clinical manifestation of oropharyngeal candidiasis in HIV-positive
       patients. We measured the secretory proteinase activities of clinical C.
       albicans isolates from the oropharynges of either HIV-positive
       individuals (n = 100) or a control group (n = 122). The mean secretory
       proteinase activity of C. albicans isolates from the HIV-positive group
       (4,255 +/- 2,372 U/liter) was significantly higher compared with that of
       isolates from the control group (2,324 +/- 1,487 U/liter) (P < 0.05).
       The higher level of secretory proteinase activity in the culture
       supernatants of individual C. albicans isolates correlated with the
       increased level of proteinase expression on the cell surface, as
       revealed by cytofluorometry, and with higher levels of secretion of the
       immunodetectable protein, as shown by Western blotting (immunoblotting).
       Proteinase activity within the population of C. albicans isolates from
       HIV-positive individuals was independent of the patient's clinical
       disease stage and the CD4+/CD8+ cell numbers. Furthermore, no
       correlation of the proteinase activities with the C. albicans serotype
       was found, although C. albicans serotype B was significantly more
       frequent in the HIV-positive group (40%) compared with that in the
       control group (12%). However, a positive correlation of proteinase
       activity to antifungal susceptibility was evident.(ABSTRACT TRUNCATED AT
       250 WORDS)
 DE    Adult  Age Factors  Aged  Antifungal Agents/PHARMACOLOGY  Blotting,
       Western  Candida albicans/IMMUNOLOGY/ISOLATION & PURIF/*PATHOGENICITY
       Candidiasis, Oral/*ENZYMOLOGY/ETIOLOGY/IMMUNOLOGY  Comparative Study
       Female  Flow Cytometry  Human  HIV Infections/*COMPLICATIONS
       Immunoassay  Male  Microbial Sensitivity Tests  Middle Age  Peptide
       Peptidohydrolases/*SECRETION  Serotyping  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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