Document 0163
 DOCN  M9650163
 TI    Strand displacement synthesis of the long terminal repeats by HIV
       reverse transcriptase.
 DT    9605
 AU    Fuentes GM; Rodriguez-Rodriguez L; Palaniappan C; Fay PJ; Bambara RA;
       Department of Microbiology & Immunology, University of Rochester,;
       School of Medicine and Dentistry, New York 14642, USA.
 SO    J Biol Chem. 1996 Jan 26;271(4):1966-71. Unique Identifier : AIDSLINE
       MED/96147167
 AB    According to the current model for retroviral replication, strand
       displacement of the long terminal repeat (LTR) is a necessary step
       during plus strand DNA synthesis in vivo. We have investigated the
       ability of human immunodeficiency virus reverse transcriptase (HIV-RT)
       to synthesize in vitro over a 634-nucleotide HIV LTR DNA template,
       having or lacking a single full-length DNA downstream primer. The
       presence of the downstream primer resulted in an approximately 12-fold
       reduction in the rate of upstream primer elongation. Addition of
       Escherichia coli single-stranded binding protein (SSB) or human
       replication protein A (RP-A) enhanced strand displacement synthesis;
       however, addition of HIV nucleocapsid protein (NC) did not. The presence
       of excess single-stranded DNA complementary to the downstream primer did
       not stimulate displacement synthesis. Interestingly, we observed that
       the elongating upstream primer could readily transfer to this DNA. This
       observation suggests that recombination is favored during strand
       displacement synthesis in vivo.
 DE    Base Sequence  DNA Primers/CHEMISTRY  DNA-Binding Proteins/METABOLISM
       DNA, Single-Stranded/METABOLISM  DNA, Viral/*BIOSYNTHESIS  Human  HIV
       Long Terminal Repeat/*GENETICS  HIV-1/*ENZYMOLOGY/GENETICS  Molecular
       Sequence Data  RNA-Directed DNA Polymerase/*METABOLISM  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Templates  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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