Document 0164
 DOCN  M9650164
 TI    Activation of lavage lymphocytes in lung injuries caused by radiotherapy
       for lung cancer.
 DT    9605
 AU    Nakayama Y; Makino S; Fukuda Y; Min KY; Shimizu A; Ohsawa N; First
       Department of Internal Medicine, Osaka Medical College,; Japan.
 SO    Int J Radiat Oncol Biol Phys. 1996 Jan 15;34(2):459-67. Unique
       Identifier : AIDSLINE MED/96158778
 AB    PURPOSE: Radiation pneumonitis sometimes extends beyond the irradiated
       area of a lung and can also affect the opposite lung. Some immunological
       mechanisms, in addition to simple direct injury of the lungs by
       radiation, seem to be involved in the onset of radiation pneumonitis. To
       clarify such mechanisms, the effects of radiation on local inflammatory
       cells in lungs, in particular, lymphocytes, were examined. METHODS AND
       MATERIALS: A comparison was made of bronchoalveolar lavage fluid (BALF)
       findings from 13 irradiated patients (RT group) and 15 nonirradiated
       patients (non-RT group) with lung cancer. Patients who later developed
       radiation pneumonitis (RP group) and those who did not (RP-free group)
       were also compared. Using a two-color flowcytometer, radiation-induced
       changes in local inflammatory cells in lungs were analyzed. This
       included analyses of human leukocyte-associated antigen (HLADR) and
       intercellular adhesion molecule-1 (ICAM-1) expression on T-cells, which
       are though to be involved in cell activation and interactions between
       cells. RESULTS: The following aspects of BALF were higher in the RT
       group than in the non-RT group: (a) the percentage of lymphocytes and
       eosinophiles; (b) the incidence of HLADR-positive CD4+T-cells and
       HLADR-positive CD8+T-cells; and (c) the incidence of ICAM-1--positive
       T-cells. The following aspects of BALF were higher in the RP group than
       in the RP-free group: (a) the total cell counts; (b) the percentage of
       lymphocytes; and (c) the incidence of ICAM-1-positive T-cells. A
       significant relationship was seen between the incidence of ICAM-1
       expression on T-cells and the number of days from the initiation of
       radiotherapy to the onset of radiation pneumonitis. CONCLUSION: These
       data suggest that irradiation can induce accumulation of activated
       T-cells (HLADR and ICAM-1--positive T-cells) in the lung. This
       accumulation may be closely linked to radiation-induced lung injury. It
       is also suggested that the incidence of ICAM-1--positive T-cells in BALF
       may serve as a useful clinical marker of radiation pneumonitis.
 DE    Adult  Aged  Bronchoalveolar Lavage Fluid/*CYTOLOGY  Carcinoma,
       Non-Small-Cell Lung/PATHOLOGY/*RADIOTHERAPY  Carcinoma, Small
       Cell/PATHOLOGY/*RADIOTHERAPY  CD4-Positive
       T-Lymphocytes/CHEMISTRY/*RADIATION EFFECTS  CD8-Positive
       T-Lymphocytes/CHEMISTRY/*RADIATION EFFECTS  Female  Human  HLA-DR
       Antigens/*ANALYSIS  Intercellular Adhesion Molecule-1/*ANALYSIS  Lung
       Neoplasms/PATHOLOGY/*RADIOTHERAPY  Lymphocyte Transformation  Male
       Middle Age  Radiation Pneumonitis/*IMMUNOLOGY  JOURNAL ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).