Document 0173
 DOCN  M9650173
 TI    Major histocompatibility complex class I presentation of exogenous and
       endogenous protein-derived peptides by a transfected human monocyte cell
       line.
 DT    9605
 AU    Harris PE; Colovai AI; Maffei A; Liu Z; Foca NS; Department of
       Pathology, College of Physicians and Surgeons of; Columbia University,
       New York, NY, USA.
 SO    Immunology. 1995 Dec;86(4):606-11. Unique Identifier : AIDSLINE
       MED/96165039
 AB    Monocyte/macrophages are professional antigen-presenting cells of the
       cellular immune system, serving to generate peptides for major
       histocompatibility complex (MHC) class II-restricted recognition by CD4+
       T-lymphocyte effector cells. Antigen presentation by these cells
       involves the internalization of extracellular proteins and their
       fragmentation within vacuolar compartments. The resulting peptides
       become associated with MHC class II molecules. The final destination of
       exogenous peptide antigens, however, is not absolute in monocytes.
       Processed peptides, derived from exogenous proteins, can also associate
       with MHC class I molecules. To study simultaneous presentation of
       peptides derived from exogenous and endogenous proteins by human
       leucocyte antigen (HLA) class I molecules, we isolated the peptides from
       a human immunodeficiency virus nef transfected U937 monocytic cell line.
       The HLA class I-bound peptides were separated by reverse phase-high
       performance liquid chromatography. Comparison of the peptide sequence
       data with protein databases revealed that the peptides derived from
       extracellular, as well as intracellular, proteins, suggesting that
       monocytes have a more generalized MHC class I antigen-processing pathway
       than previously documented.
 DE    Amino Acid Sequence  Antigen Presentation/*IMMUNOLOGY  Antigens,
       CD4/METABOLISM  Chromatography, High Pressure Liquid  Gene Products,
       nef/METABOLISM  Histocompatibility Antigens Class I/*IMMUNOLOGY  Human
       HIV  Molecular Sequence Data  Monocytes/*IMMUNOLOGY
       Peptides/CHEMISTRY/*IMMUNOLOGY  Polymerase Chain Reaction  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Transfection  Tumor Cells,
       Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).