Document 0188
 DOCN  M9650188
 TI    Decreased interleukin-2 production in murine acute pancreatitis:
       potential for immunomodulation [see comments]
 DT    9605
 AU    Curley P; Nestor M; Collins K; Saporoschetz I; Mendez M; Mannick JA;
       Rodrick ML; Department of Surgery, Brigham and Women's Hospital,
       Boston,; Massachusetts, USA.
 SO    Gastroenterology. 1996 Feb;110(2):583-8. Unique Identifier : AIDSLINE
       MED/96156062
 CM    Comment in: Gastroenterology 1996 Feb;110(2):639-42
 AB    BACKGROUND & AIMS: The role of the cytokine interleukin 2 (IL-2) has
       long been recognized as central to normal immunologic function and
       defense against infection after burns and trauma, but little effort has
       been directed towards its role in acute pancreatitis (AP), which also
       has a high mortality related to sepsis. This study investigated the
       potential role of IL-2 in mice with diet-induced AP. METHODS: AP was
       induced in mice by 10 days of feeding a choline-deficient,
       ethionine-supplemented diet. T-helper (CD4) cells were estimated, and
       T-cell mitogen-stimulated splenocyte proliferation and IL-2 production
       in vitro were measured on days 3, 7, and 10. RESULTS: Significant
       reduction in IL-2 production was found on day 3 (32%; P < 0.05) and day
       10 (48%; P < 0.005). Administration of intraperitoneal
       lipopolysaccharide on day 10 was associated with reduced IL-2 production
       (P < 0.025) 4 hours later and 90% mortality in animals with AP. In vivo
       therapy with recombinant IL-2 improved in vitro IL-2 secretion (P <
       0.05) and reduced lipopolysaccharide-induced mortality (P = 0.036).
       CONCLUSIONS: Murine diet-induced AP is associated with impaired immune
       function and increased susceptibility to sepsis and may be a valuable
       tool in the investigation of immunomodulation in AP.
 DE    Acute Disease  Adjuvants, Immunologic  Animal  CD4 Lymphocyte Count
       Endotoxins/ADVERSE EFFECTS  Female
       Interleukin-2/*BIOSYNTHESIS/THERAPEUTIC USE  Lipopolysaccharides/ADVERSE
       EFFECTS  Lymphocyte Transformation  Mice  Mice, Inbred Strains
       Pancreatitis/*IMMUNOLOGY/METABOLISM/THERAPY  Recombinant
       Proteins/THERAPEUTIC USE  Support, Non-U.S. Gov't
       T-Lymphocytes/IMMUNOLOGY/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).