Document 0193
 DOCN  M9650193
 TI    Default development of cloned human naive CD4 T cells into
       interleukin-4- and interleukin-5- producing effector cells.
 DT    9605
 AU    Yang LP; Byun DG; Demeure CE; Vezzio N; Delespesse G; University of
       Montreal, Louis-Charles Simard Research Center,; Notre-Dame Hospital,
       Montreal, Canada.
 SO    Eur J Immunol. 1995 Dec;25(12):3517-20. Unique Identifier : AIDSLINE
       MED/96140696
 AB    It was recently demonstrated that naive human and mouse CD4 T cells
       release low but sufficient levels of interleukin (IL)-4 at priming to
       support their development into IL-4 producers. To determine whether this
       IL-4 is produced by a minor subset of cells, freshly isolated human
       naive CD4 T cells were directly cloned by limiting dilution in the
       absence of exogenous IL-4. More than 95% of neonatal and 60% of adult
       naive T cells seeded in single-cell cultures could be expanded upon
       stimulation with anti-CD3 mAb immobilized on CD32-B7.1 L cell
       transfectants in the presence of IL-2. All 171 clones derived from four
       neonates and two adults produced IL-4 and IL-5 at generally high levels.
       Like the allergen-specific human Th2 clones described in the literature,
       these T cell clones produced little or no interferon-gamma upon
       stimulation via their T cell receptor/CD3 complex, whereas they released
       high levels of this cytokine when activated with phorbol 12-myristate
       13-acetate+ionomycin. Cells cloned and expanded in the presence of
       anti-IL4 + anti-IL-4R mAb produced much lower levels of IL-4 and IL-5.
       It is concluded that almost every single naive human CD4 T cell primed
       and expanded in the absence of exogenous IL-4 releases sufficient
       autocrine IL-4 to support its clonal expansion into high IL-4/IL-5
       producers.
 DE    Adult  Cell Differentiation/IMMUNOLOGY  Clone Cells  CD4-Positive
       T-Lymphocytes/CYTOLOGY/*METABOLISM  Fetal Blood/IMMUNOLOGY  Human
       Infant, Newborn  Interleukin-4/*BIOSYNTHESIS
       Interleukin-5/*BIOSYNTHESIS  Lymphocyte Transformation  Support,
       Non-U.S. Gov't  Th2 Cells/CYTOLOGY/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).