Document 0197
 DOCN  M9650197
 TI    Altered Th1/Th2 balance associated with the immunosuppressive/protective
       effect of the H-2Ab allele on the response to
       allo-4-hydroxyphenylpyruvate dioxygenase.
 DT    9605
 AU    Brunner M; Larsen S; Sette A; Mitchison A; Deutsches
       Rheuma-Forschungszentrum, Berlin, Germany.
 SO    Eur J Immunol. 1995 Dec;25(12):3285-9. Unique Identifier : AIDSLINE
       MED/96140664
 AB    The H-2Ab allele exerts a dominant down-regulatory effect on the
       anti-allo-HPPD (4-hydroxyphenylpyruvate dioxygenase) antibody response,
       through a hitherto unknown mechanism. In the present study, the
       allo-variable peptide bound to responder H-2Ak molecules with higher
       affinity than to H-2Ab ones, arguing against the operation of an
       affinity hierarchy. Quantitative polymerase chain reaction revealed
       differences in cytokine mRNA expression between suppressed and
       high-responder mice. Lymph node cells of responder but not suppressed
       mice contained high levels of interleukin (IL)-4 mRNA as early as 11 h
       post-immunization and continued to do so for at least 8 days; this early
       burst was paralleled by a small burst in transforming growth factor
       (TGF)-beta mRNA level. Differences in IL-12 mRNA were not detected,
       although an early IL-12 effect could not be excluded. Interferon
       (IFN)-gamma appeared to contribute to the suppression at later time
       points. Early treatment of responder mice with anti-IL-4 monoclonal
       antibody (11B11) down-regulated the antibody response. The proliferative
       T cell response from hyperimmunized mice was reduced but still
       detectable in the presence of an H-2Ab allele. Thus, in the presence of
       this allele, the Th1 response is enhanced and that of Th2 cells
       suppressed, apparently as a result of the bias of H-2Ab-restricted T
       cells in favor of the Th1 subset.
 DE    *Alleles  Animal  *Antibody Formation  Base Sequence
       Cytokines/BIOSYNTHESIS  Female  H-2 Antigens/GENETICS/*IMMUNOLOGY
       Immunophenotyping  *Immunosuppression  Interleukin-4/IMMUNOLOGY  Mice
       Mice, Inbred CBA  Molecular Sequence Data  Protein Binding/IMMUNOLOGY
       RNA, Messenger/BIOSYNTHESIS  Support, Non-U.S. Gov't  Th1
       Cells/*IMMUNOLOGY  Th2 Cells/*IMMUNOLOGY  4-Hydroxyphenylpyruvate
       Dioxygenase/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).