Document 0215
 DOCN  M9650215
 TI    T cell clones from a Sjogren's syndrome salivary gland biopsy produce
       high levels of IL-10.
 DT    9605
 AU    Brookes SM; Cohen SB; Price EJ; Webb LM; Feldmann M; Maini RN; Venables
       PJ; Kennedy Institute of Rheumatology, London, UK.
 SO    Clin Exp Immunol. 1996 Feb;103(2):268-72. Unique Identifier : AIDSLINE
       MED/96152675
 AB    Sjogren's syndrome (SS) is characterized by a focal periductal salivary
       gland infiltrate consisting mainly of T and B lymphocytes. Most of the T
       cells bear the memory CD4+ Th1-like phenotype and express high levels of
       class II, though CD8+ cells are also present. We have studied 17 labial
       salivary gland and 15 peripheral blood T cell clones from a patient with
       primary SS. The tissue clones were 71% CD8+ and 29% CD4+, and the
       peripheral blood-derived clones were 60% CD8+ and 40% CD4+. The CD4+ T
       cell clones from both the salivary gland and autologous peripheral blood
       were of the Th1 phenotype, in that they produced interferon-gamma
       (IFN-gamma) and IL-2 but very little IL-4 after 24 h stimulation with
       phorbol myristate acetate and anti-CD3 antibody. The salivary
       gland-derived CD4+ clones produced 15 times more IL-10 (7.92 ng/ml) than
       peripheral blood-derived CD4+ clones (0.52 ng/ml, P < or = 0.02). The
       tissue CD8+ clones produced 1.2 times (P < 0.04) more IFN-gamma and CD4+
       clones produced 3.5 times less IL-2 (P < 0.02) than the respective
       PBM-derived clones. The accumulation of Th1-type cells producing high
       levels of IL-10 in the salivary gland suggests a specific
       immunoregulatory function at the site of inflammation in SS.
 DE    Antigens, CD4/IMMUNOLOGY  Antigens, CD8/IMMUNOLOGY  Biopsy  Case Report
       Clone Cells  Female  Human  Interleukin-10/*BIOSYNTHESIS  Middle Age
       Salivary Glands/*PATHOLOGY  Sjogren's Syndrome/*IMMUNOLOGY/PATHOLOGY
       Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY/PATHOLOGY  Th1
       Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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