Document 0218
 DOCN  M9650218
 TI    Interferon-gamma (IFN-gamma) treatment decreases the inflammatory
       response in chronic Pseudomonas aeruginosa pneumonia in rats.
 DT    9605
 AU    Johansen HK; Hougen HP; Rygaard J; Hoiby N; Department of Clinical
       Microbiology, Rigshospitalet, Copenhagen,; Denmark.
 SO    Clin Exp Immunol. 1996 Feb;103(2):212-8. Unique Identifier : AIDSLINE
       MED/96152667
 AB    In a rat model of chronic Pseudomonas aeruginosa lung infection
       mimicking cystic fibrosis (CF), we studied whether the inflammatory
       response could be altered by intraperitoneal treatment with recombinant
       rat interferon-gamma (rrIFN-gamma). Rats were treated either before or
       after intratracheal challenge with P. aeruginosa embedded in alginate
       beads. Rats treated after challenge had a significant reduction in the
       severity of macroscopic lung inflammation compared with rats treated
       before challenge (P = 0.004) and controls (P = 0.003). The
       histopathology in controls was dominated by numerous polymorphonuclear
       leucocytes (PMN) (> or = 90%) surrounding the alginate beads like in CF.
       This could be caused by a Th2-like response. In contrast, a complete
       shift to a chronic-type inflammation dominated by mononuclear leucocytes
       (> or = 90% lymphocytes and plasma cells) and granulomas was observed in
       both rrIFN-gamma-treated groups of rats. This could be caused by a
       Th1-like response. There was no significant difference in lethality
       between the groups, and the antibody titres against P. aeruginosa
       sonicate and alginate were similar in the treated rats and controls.
       Since the ongoing lung tissue damage in CF patients has been shown to be
       caused by elastase secreted by PMN, which dominate the P. aeruginosa
       lung infection, our findings offer a possible new strategy of modifying
       the inflammatory response in CF patients.
 DE    Animal  Cystic Fibrosis/IMMUNOLOGY/PATHOLOGY/THERAPY  Disease Models,
       Animal  Female  Inflammation/*THERAPY  Interferon-gamma,
       Recombinant/*THERAPEUTIC USE  Pneumonia,
       Bacterial/IMMUNOLOGY/PATHOLOGY/*THERAPY  Pseudomonas
       Infections/IMMUNOLOGY/PATHOLOGY/*THERAPY  Rats  Rats, Inbred Lew
       Support, Non-U.S. Gov't  Th1 Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).