Document 0223
 DOCN  M9650223
 TI    Mitogenic effect of HIV-infected human T cell lines on mouse B cells
       mediated by surface immunoglobulin.
 DT    9605
 AU    Arase N; Arase H; Ohki K; Nishino Y; Ikuta K; Onoe K; Section of
       Pathology, Hokkaido University, Sapporo, Japan.
 SO    Clin Exp Immunol. 1996 Jan;103(1):24-9. Unique Identifier : AIDSLINE
       MED/96150277
 AB    Following HIV-1 infection, a number of disorders are induced in both
       normal T and B cells by virus products derived from infected CD4+ T
       cells. In the present study, we found that HIV-infected, but not
       uninfected, human T cell lines generated vigorous blastogenesis and
       proliferation of freshly isolated mouse B cells in a short-term culture.
       Neither human B cells nor rat B cells showed significant responses to
       the HIV-infected T cell lines in the present condition. The mitogenic
       effect of HIV-infected human T cell line requires direct cell-cell
       interaction between mouse B cells and HIV-infected T cell lines. Since
       either mitomycin c treatment or paraformaldehyde fixation of
       HIV-infected T cell lines resulted in complete loss of the mitogenic
       effect, it seems that de novo synthesized viral products are responsible
       for this effect. Furthermore, anti-mouse immunoglobulin antibody
       inhibited completely the B cell stimulation by the HIV-infected human T
       cell lines. Thus, surface immunoglobulin (sIg) on mouse B cells appears
       to be an essential molecule which transduces activation signals from
       HIV-infected human T cells into cytoplasm of the B cells.
 DE    Animal  B-Lymphocytes/IMMUNOLOGY/*VIROLOGY  Cell Communication/DRUG
       EFFECTS/IMMUNOLOGY  Human  HIV-1/*IMMUNOLOGY  Immunoglobulins,
       Surface/*PHARMACOLOGY  Leukemia, T-Cell  *Lymphocyte Transformation/DRUG
       EFFECTS  Mice  Mice, Inbred C57BL  Mitosis/DRUG EFFECTS/*IMMUNOLOGY
       Support, Non-U.S. Gov't  T-Lymphocytes/IMMUNOLOGY/*VIROLOGY  Tumor
       Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).