Document 0233
 DOCN  M9650233
 TI    Generation of human T lymphocytes from bone marrow CD34+ cells in vitro.
 DT    9605
 AU    Freedman AR; Zhu H; Levine JD; Kalams S; Scadden DT; Division of
       Hematology/Oncology, New England Deaconess Hospital,; Harvard Medical
       School, Boston, Massachusetts 02129, USA.
 SO    Nat Med. 1996 Jan;2(1):46-51. Unique Identifier : AIDSLINE MED/96135164
 AB    Analysis of the events that regulate development of red blood cells or
       granulocytes has led to therapies altering clinical conditions
       associated with anemia or neutropenia. The development of therapeutic
       approaches to target conditions associated with lymphopenia, such as
       AIDS, has been thwarted by limited techniques for studying T-lymphocyte
       development. We describe an in vitro system in which human bone marrow
       CD34+ cells proliferate, acquire the expression of the lymphoid-specific
       RAG-2 gene and a broad repertoire of rearranged T-cell receptor genes,
       develop the ability to produce T cell-specific interleukin-2 and achieve
       a range of T-cell immunophenotypes. The cells also become susceptible to
       infection with the T-lymphotropic strain of human immunodeficiency
       virus-1, HIV-1IIIB. This culture system induces human T lymphopoiesis
       and may permit further analysis of the events regulating human T-lineage
       differentiation. It provides a preclinical model for screening stem cell
       gene therapies directed toward AIDS.
 DE    Adult  Antigens, CD  *Antigens, CD34  Base Sequence  Bone
       Marrow/*CYTOLOGY/*IMMUNOLOGY  Cells, Cultured  DNA Primers  Fetus  Flow
       Cytometry  Gene Expression  Hematopoietic Stem Cells/CYTOLOGY/IMMUNOLOGY
       Human  HIV-1/*PHYSIOLOGY/PATHOGENICITY  Immunophenotyping
       Interleukin-2/BIOSYNTHESIS  Molecular Sequence Data  Polymerase Chain
       Reaction  Proteins/BIOSYNTHESIS  Receptors, Antigen, T-Cell,
       alpha-beta/GENETICS  Stromal Cells/CYTOLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  T-Lymphocytes/CYTOLOGY/*IMMUNOLOGY/VIROLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).