Document 0250
 DOCN  M9650250
 TI    Inflammatory and T-cell profile of asthmatic airways 6 hours after local
       allergen provocation.
 DT    9605
 AU    Gratziou C; Carroll M; Montefort S; Teran L; Howarth PH; Holgate ST;
       University Medicine, Southampton General Hospital, United; Kingdom.
 SO    Am J Respir Crit Care Med. 1996 Feb;153(2):515-20. Unique Identifier :
       AIDSLINE MED/96160556
 AB    T cells in the airways are considered to play a key role in
       orchestrating the inflammatory response of asthma through the
       elaboration of specific cytokines. Using flow cytometry we have
       investigated the T-cell response of sensitized asthmatic airways 6 h
       after local allergen provocation. Twelve subjects with atopic asthma
       underwent bronchoalveolar lavage (BAL) before and 6 h after local
       instillation of allergen into the right middle lobe (RML) and saline
       into the right upper lobe (RUL). Allergen challenge produced a
       significant 26% fall in FEV1, an increase in eosinophils in BAL at 6 h,
       and at 24 h an increase in methacholine responsiveness compatible with
       late-phase airway inflammation. When compared with saline challenge,
       allergen produced an overall decrease in the number of BAL lymphocytes
       from 21.3 +/- 2.8% to 16.0 +/- 3.08% of total cells but no change in the
       proportion of CD4+, CD8+, CD25+, or HLA-DR+ cells. Allergen provocation
       reduced the proportion of T cells expressing the beta 2 integrin
       lymphocyte functional antigen-1 (LFA-1) from 72.5 +/- 30 to 43.9 +/- 9.1
       mean fluorescent units (p < 0.01) and a similar trend in intercellular
       adhesion molecule-1 (ICAM-1) (p = 0.08). These results indicate that
       late-phase inflammatory events 6 h after local allergen provocation
       involve the selective retention of airway T cells expressing specific
       cell adhesion molecules.
 DE    Adult  *Allergens  Antigens, CD/ANALYSIS
       Asthma/*IMMUNOLOGY/PATHOLOGY/PHYSIOPATHOLOGY
       Bronchi/*IMMUNOLOGY/PATHOLOGY  Bronchial Provocation Tests
       Bronchoalveolar Lavage Fluid/IMMUNOLOGY  Bronchoscopy  Cell Count  CD4
       Lymphocyte Count  CD8-Positive T-Lymphocytes  Female  Flow Cytometry
       Forced Expiratory Volume  Human  HLA-DR Antigens/ANALYSIS  Inflammation
       Intercellular Adhesion Molecule-1/METABOLISM  Lymphocyte Count  Male
       Support, Non-U.S. Gov't  *T-Lymphocyte Subsets  T-Lymphocytes/METABOLISM
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).