Document 0260
 DOCN  M9650260
 TI    Morphological studies on avian spinal cord chimeras.
 DT    9605
 AU    Saito K; Sugisaki T; Yang G; Milgrom F; Albini B; Department of
       Microbiology, State University of New York at; Buffalo 14214, USA.
 SO    Int Arch Allergy Immunol. 1996 Feb;109(2):116-26. Unique Identifier :
       AIDSLINE MED/96162072
 AB    Spinal cord chimeras were constructed by orthotopic grafting of quail
       embryonal neutral folds, neural crest and neural tube into chicken
       embryos. The spinal cord xenografts were accepted for varying lengths of
       time, but most chimeras eventually rejected the quail transplant. This
       was associated with perivenular cuffing and demyelination with
       preservation of most neurons, as well as clinical neurological symptoms.
       Twenty-four chimeras were studied to delineate the time of first
       appearance of glial deposits of immunoglobulin and to identify the
       subpopulations of T cells in spinal cord infiltrates. The results
       suggested that deposits of immunoglobulins on glial elements preceded
       inflammatory cell infiltration. The perivenular cuffs consisted
       predominantly of T cells and showed a preponderance of CD8- over
       CD4-positive cells (CD4/CD8 ratios around 0.6). Further, CD4+ cells were
       found almost exclusively in the central portions of the infiltrate, with
       the periphery consisting almost only of CD8+ cells. The diffuse cellular
       infiltrate of the parenchyme contained T and plasma cells. The T cells
       were almost exclusively CD8+. Plasma cells were seen only at the outer
       borders of the cuffs and dispersed throughout the quail-derived spinal
       cord tissue. It seemed that rejection of quail-derived melanocytes in
       feathers ('quail-like feathers'), described by us earlier, often
       preceded neurological symptoms and showed a histopathological pattern
       comparable to spinal cord lesions, i.e., predominantly perivascular
       cuffing. In preliminary studies, enhancement of disease by immunization
       with quail organ suspension and decreased intensity of disease by
       combined immunosuppressive treatment with FK 506 and cycylophosphamide
       were suggested. The data presented here are compatible with the
       hypothesis that rejection of CNS quail tissue by chimeras is preceded in
       the periphery by rejection of melanocytes in segments of skin and in
       feathers, and that the spinal cord rejection relies on xenoantibodies
       and on cytotoxic as well as delayed hypersensitivity-type T cells.
       Finally, these data strengthen the analogy between the histopathologic
       presentation and immune effector composition of the xenograft rejection
       lesions in the chimeras and the plaques seen in patients with multiple
       sclerosis.
 DE    Animal  Antibodies/ANALYSIS  Antibody Formation  Cell Count  Chick
       Embryo  Chickens  Chimera/DRUG EFFECTS/IMMUNOLOGY/*PHYSIOLOGY  Coturnix
       CD4-Positive T-Lymphocytes/CYTOLOGY  CD8-Positive T-Lymphocytes/CYTOLOGY
       Erythrocytes/IMMUNOLOGY  Female  IgG/METABOLISM  Immunosuppressive
       Agents/PHARMACOLOGY  Male  Melanocytes/CYTOLOGY  Nervous System
       Diseases/ETIOLOGY  Plasma Cells  Spinal Cord/*ANATOMY &
       HISTOLOGY/IMMUNOLOGY/PATHOLOGY  Support, Non-U.S. Gov't  T-Lymphocyte
       Subsets  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).