Document 0261
 DOCN  M9650261
 TI    Assignment and modeling of the Rev Response Element RNA bound to a Rev
       peptide using 13C-heteronuclear NMR.
 DT    9605
 AU    Battiste JL; Tan R; Frankel AD; Williamson JR; Department of Chemistry,
       Massachusetts Institute of Technology,; Cambridge 02139, USA.
 SO    J Biomol NMR. 1995 Dec;6(4):375-89. Unique Identifier : AIDSLINE
       MED/96141450
 AB    The Rev Response Element (RRE) RNA-Rev protein interaction is important
       for regulation of gene expression in the human immunodeficiency virus. A
       model system for this interaction, which includes stem IIB of the RRE
       RNA and an arginine-rich peptide from the RNA-binding domain of Rev, was
       studied using multidimensional heteronuclear NMR. Assignment of the RNA
       when bound to the peptide was obtained from NMR experiments utilizing
       uniformly and specifically 13C-labeled RNA. Isotopic filtering
       experiments on the specifically labeled RNA enable unambiguous
       assignment of unusual nonsequential NOE patterns present in the internal
       loop of the RRE. A three-dimensional model of the RNA in the complex was
       obtained using restrained molecular dynamics calculations. The internal
       loop contains two purine-purine base pairs, which are stacked to form
       one continuous helix flanked by two A-form regions. The formation of a
       G-G base pair in the internal loop requires an unusual structure of the
       phosphate backbone. This structural feature is consistent with
       mutational data as being important for the binding of Rev to the RRE.
       The G-G base pair may play an important role in opening the normally
       narrow major groove of A-form RNA to permit binding of the Rev basic
       domain.
 DE    Gene Products, rev/GENETICS/*METABOLISM  Genes, env/*GENETICS  Human
       HIV/*GENETICS  Models, Molecular  Nuclear Magnetic Resonance  Nucleic
       Acid Conformation  Protein Binding  Protein Conformation  RNA,
       Viral/*GENETICS/METABOLISM  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).