Document 0270
 DOCN  M9650270
 TI    Detection of Pneumocystis carinii, Mycobacterium tuberculosis, and
       cytomegalovirus in human immunodeficiency virus (HIV)-infected patients
       with hemophilia by polymerase chain reaction of induced sputum samples.
 DT    9605
 AU    Shimomoto H; Hasegawa Y; Takagi N; Ichiyama S; Takamatsu J; Saito H;
       Shimokata K; First Department of Internal Medicine, Nagoya University
       School; of Medicine.
 SO    Intern Med. 1995 Oct;34(10):976-81. Unique Identifier : AIDSLINE
       MED/96157629
 AB    The purpose of this study was to diagnose the initial stage of acute
       pulmonary infections due to Pneumocystis carinii (P. carinii),
       Mycobacterium tuberculosis (M. tuberculosis), and cytomegalovirus (CMV)
       by polymerase chain reaction (PCR) combined with the induced sputum
       method in hemophilia patients infected with human immunodeficiency virus
       (HIV). Induced sputum and PCR were obtained at each outpatient visit or
       when any respiratory symptoms were present during a one-year period. All
       patients with a CD4+ count of less than 200 x 10(6)/l were given
       prophylactics against P. carinii pneumonia (PCP). PCR was conducted 111
       times in 29 patients. M. tuberculosis DNA was detected in 1 patient and
       CMV DNA in 4 patients. P. carinii DNA was not detected. PCR on induced
       sputum samples is sensitive for the rapid detection of the initial stage
       of respiratory events. PCP prophylaxis changes the clinical
       manifestations of HIV infection.
 DE    Adolescence  Adult  AIDS-Related Opportunistic
       Infections/*DIAGNOSIS/MICROBIOLOGY  Base Sequence
       Cytomegalovirus/*ISOLATION & PURIF  Cytomegalovirus
       Infections/*DIAGNOSIS/VIROLOGY  CD4 Lymphocyte Count  DNA,
       Bacterial/ANALYSIS  DNA, Fungal/ANALYSIS  DNA, Viral/ANALYSIS
       Hemophilia/*COMPLICATIONS  Human  Male  Molecular Sequence Data
       Mycobacterium tuberculosis/*ISOLATION & PURIF  Oxygen/BLOOD
       Pneumocystis carinii/*ISOLATION & PURIF  Pneumonia, Pneumocystis
       carinii/*DIAGNOSIS/MICROBIOLOGY  Polymerase Chain Reaction
       Sputum/*MICROBIOLOGY  Tuberculosis/DIAGNOSIS/MICROBIOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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