Document 0292
 DOCN  M9650292
 TI    Cross-linking of Fc gamma receptors activates HIV-1 long terminal
       repeat-driven transcription in human monocytes.
 DT    9605
 AU    Tsitsikov EN; Fuleihan R; McIntosh K; Scholl PR; Geha RS; Division of
       Immunology, Children's Hospital, Boston, MA 02115,; USA.
 SO    Int Immunol. 1995 Oct;7(10):1665-70. Unique Identifier : AIDSLINE
       MED/96128660
 AB    Elevation of the levels of circulating immune complexes frequently
       accompanies HIV-1 infection and is a prognostic indicator of clinical
       progression from asymptomatic infection to AIDS. Here we report that
       cross-linking of Fc gamma RI or Fc gamma RII by adherent human IgG or by
       specific anti-Fc gamma R mAb activates HIV-1 gene expression in the
       human monocytic cell line BF24 and increased HIV RNA expression in
       monocytes from HIV infected patients as assayed by reverse
       transcription-PCR. In THP-1 cells, Fc gamma R cross-linking induced
       NF-kappa B, which is known to bind to the regulatory region of the long
       terminal repeat (LTR) of HIV-1 and to activate HIV-1 transcription.
       Anti-TNF-alpha antibody but not anti-IL-1 beta antibody strongly
       inhibited both the induction of HIV-1-LTR-driven transcription and the
       induction of NF-kappa B by Fc gamma R cross-linking. These results
       indicate that Fc gamma R can mediate a TNF-alpha-dependent induction of
       HIV-1 gene transcription and suggest that immune complexes may
       contribute to the pathophysiology of HIV-1 infection by augmenting viral
       replication in monocytes.
 DE    Acquired Immunodeficiency Syndrome/*BLOOD/IMMUNOLOGY/VIROLOGY
       Antibodies, Monoclonal/IMMUNOLOGY  Antigen-Antibody Complex/*IMMUNOLOGY
       Base Sequence  Chloramphenicol Acetyltransferase/BIOSYNTHESIS/GENETICS
       *Gene Expression Regulation, Viral  Genes, Reporter  Human  *HIV Long
       Terminal Repeat  HIV-1/GENETICS/PHYSIOLOGY  IgG/*IMMUNOLOGY
       Immunoglobulins, Fab/IMMUNOLOGY  *Immunologic Capping  Leukemia,
       Monocytic, Acute/PATHOLOGY  Molecular Sequence Data
       Monocytes/*IMMUNOLOGY/VIROLOGY  NF-kappa B/METABOLISM  Receptors,
       IgG/*IMMUNOLOGY  Recombinant Fusion Proteins/BIOSYNTHESIS  RNA,
       Viral/BIOSYNTHESIS/GENETICS  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  *Transcription, Genetic  Tumor Cells, Cultured  Tumor
       Necrosis Factor/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).