Document 0326
 DOCN  M9650326
 TI    HIV-1 Tat modulates invasion by a bacterial enteric pathogen into a
       human intestinal cell line.
 DT    9605
 AU    Mayer HB; Wanke CA; Du B; Hammer SM; Terwilliger EF; Division of
       Infectious Diseases, New England Deaconess Hospital,; Boston, MA 02115,
       USA.
 SO    AIDS. 1995 Nov;9(11):1237-42. Unique Identifier : AIDSLINE MED/96126177
 AB    OBJECTIVE: To explore the possibility that an HIV-1 gene product may
       modulate entry of an invasive enteric pathogen into a terminally
       differentiated human intestinal cell line. HIV-1 Tat was selected for
       investigation because of its unique ability to cross cell membranes.
       METHODS: After transient transfection of HT29-C1 cells with plasmids
       containing HIV-1 long terminal repeat (LTR)-lacZ plus a Tat expression
       cassette, or with a pSR-lacZ control plasmid, bacterial invasion assays
       were performed on both groups of cells utilizing a clinical Salmonella
       isolate. Assays were performed concurrently on a control group of
       non-transfected cells. A second series of experiments compared bacterial
       invasion into cells transfected with the Tat expression vector alone
       versus cells transfected with either an isogenic expression vector that
       did not make Tat, or with pSR-lacZ. Finally, the ability of exogenous
       Tat protein to transactivate an HIV-1 LTR-chloramphenicol
       acetyltransferase (CAT) plasmid which had been transfected into HT29-C1
       cells and to modulate Salmonella invasion was also assessed. RESULTS:
       HT29-C1 cells transfected with a Tat expression vector, either alone or
       in combination with another plasmid, were significantly less susceptible
       to bacterial invasion than cells that either did not undergo
       transfection, were transfected with an otherwise isogenic expression
       vector without Tat, or transfected with an unrelated plasmid. Duplicate
       experiments also demonstrated that exogenous purified Tat protein
       transactivated an HIV-1 LTR-CAT plasmid which had been transfected into
       HT29-C1 cells and inhibited Salmonella invasion compared with unexposed
       cells. CONCLUSION: HIV-1 Tat inhibits Salmonella invasion of a human
       enterocyte cell line whether the protein is expressed intracellularly or
       provided exogenously.
 DE    Animal  Cell Line  Chloramphenicol Acetyltransferase/METABOLISM  Enzyme
       Activation  Gene Products, tat/*BIOSYNTHESIS/GENETICS  Gene Transfer
       Human  *HIV-1  Intestines/METABOLISM/*MICROBIOLOGY/VIROLOGY
       Salmonella/*PATHOGENICITY  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).