Document 0330
 DOCN  M9650330
 TI    T cell lymphoma in transgenic mice expressing the human Hsp70 gene.
 DT    9605
 AU    Seo JS; Park YM; Kim JI; Shim EH; Kim CW; Jang JJ; Kim SH; Lee WH;
       Transgenic Mice Center, Cancer Research Institute, Seoul, Korea.
 SO    Biochem Biophys Res Commun. 1996 Jan 17;218(2):582-7. Unique Identifier
       : AIDSLINE MED/96144719
 AB    In the course of studying the role of hsp70 in the differentiation of
       pancreatic beta cells, transgenic founder mice were generated with the
       human hsp70 gene fused to the human insulin gene promoter. One resulted
       in a transgenic line that consistently developed diabetes mellitus, but
       unexpectedly three other independent transgenic founders developed
       generalized malignant lymphoma within 10 months after birth.
       Immunochemical and RT-PCR analyses revealed that the transgene was
       expressed in the lymphoma cells. Flow cytometric analyses revealed that
       the tumor was originated from T lymphocytes. Our results provide the
       first experimental evidence that hsp70 is involved in the tumorigenesis
       of T cells most likely through the blockage of apoptotic signals.
 DE    Animal  Base Sequence  Comparative Study  CD8-Positive
       T-Lymphocytes/PATHOLOGY  Diabetes Mellitus, Experimental/GENETICS  DNA
       Primers/CHEMISTRY  Gene Expression Regulation, Neoplastic  Heat-Shock
       Proteins 70/*GENETICS  Human  Insulin/GENETICS
       Lymphoma/*GENETICS/PATHOLOGY  Mice  Mice, Transgenic  Molecular Sequence
       Data  Promoter Regions (Genetics)  RNA, Messenger/GENETICS  Sequence
       Alignment  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).