Document 0332
 DOCN  M9650332
 TI    Use of murine subinoculation for the diagnosis and isolation of
       toxoplasmosis in HIV-infected patients with persistent lymphadenopathy.
 DT    9605
 AU    Diego JA; Vazquez JJ; Penin P; Fernandez J; Sanchez S; Gamallo C;
       Departamento de Medicina Preventiva y Salud Publica, Facultad de;
       Medicina (UAM), Madrid, Spain.
 SO    Ann Trop Med Parasitol. 1993 Apr;87(2):179-84. Unique Identifier :
       AIDSLINE MED/96170905
 AB    A murine model was used to try to elucidate the importance of Toxoplasma
       gondii infection in HIV-positive patients. Laboratory mice were
       inoculated with lymph node homogenates or buffy coats from 30
       HIV-infected individuals with persistent lymphadenopathy. Sabin and
       Feldman dye-tests, direct agglutination tests and indirect
       immunofluorescence assays (IFA) of serum samples taken from the mice six
       weeks after inoculation indicated, respectively, that 26.6%, 43.4% and
       33.3% of the lymph node inocula and 13.3%, 30.0% and 16.7% of the buffy
       coat samples had been Toxoplasma-positive. Histological examination of
       the brains of the infected mice revealed Toxoplasma tachyzoites or
       bradyzoites in the mice given buffy coat samples from one patient (3.3%)
       and those given lymph node material from three patients (10%). No
       protozoa were found in the brains of the control mice, all of which were
       sero-negative. In contrast to the mouse results, other, concurrent tests
       failed to detect any signs of toxoplasmosis infection in the patients,
       except relatively low titres of anti-Toxoplasma antibodies in 11
       patients, even though two patients developed brain toxoplasmosis, one 12
       and the other 18 months after the study. It appears, therefore, that an
       animal model can be very useful in the diagnosis of toxoplasmosis in HIV
       patients.
 DE    Adolescence  Adult  Animal  AIDS-Related Complex/*COMPLICATIONS
       Brain/PARASITOLOGY  Human  HIV Infections/*COMPLICATIONS  Male  Mice
       Toxoplasma/ISOLATION & PURIF  Toxoplasmosis, Animal/*DIAGNOSIS  JOURNAL
       ARTICLE

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