Document 0339
 DOCN  M9650339
 TI    Predominant CD8+ infiltrate in limb biopsies of individuals with
       filarial lymphedema and elephantiasis.
 DT    9605
 AU    Freedman DO; Horn TD; Maia e Silva CM; Braga C; Maciel A; Division of
       Geographic Medicine, University of Alabama at; Birmingham, USA.
 SO    Am J Trop Med Hyg. 1995 Dec;53(6):633-8. Unique Identifier : AIDSLINE
       MED/96155958
 AB    In 34 individuals with a spectrum of clinical manifestations of
       Bancroftian filariasis, we investigated whether
       immunoperoxidase-stained, random, superficial dermal biopsies could
       further elucidate the nature of the diffuse damage to superficial
       lymphatics that had been recently demonstrated by radionuclide
       lymphoscintigraphy. A total of 78% and 68% of limbs from patients with
       clinical disease and asymptomatic microfilaremia, respectively,
       contained EN4+PAL-E- lymphatic vessels that were abnormally dilated. The
       majority of subjects, regardless of clinical classification, had a CD3+
       perivascular but not a perilymphatic infiltrate in tissues and no
       parasites were present. In contrast to those individuals with
       asymptomatic infection, a striking predominance of CD8+ T cells was
       found in the tissue of individuals with clinical disease. Tissue
       pathology consistent with cutaneous bacterial infection was not
       observed. The prominent perivenular and pericapillary mononuclear
       infiltrates likely indicate, in light of current understanding of
       lymphocyte recirculation, the extravasation of lymphocytes from the
       vascular circulation into the inflamed filarial tissue.
 DE    Animal  Antibodies, Helminth/ANALYSIS  Antibodies, Monoclonal  Antigens,
       CD3/ANALYSIS  Biopsy, Needle  *Chemotaxis, Leukocyte  CD4-CD8 Ratio
       CD8-Positive T-Lymphocytes/*PATHOLOGY  Elephantiasis,
       Filarial/ETIOLOGY/*PATHOLOGY/RADIONUCLIDE IMAGING  Extremities  Human
       Immunoenzyme Techniques  Lymphatic System/PATHOLOGY/RADIONUCLIDE IMAGING
       Lymphedema/ETIOLOGY/*PATHOLOGY/RADIONUCLIDE IMAGING  Organotechnetium
       Compounds/DIAGNOSTIC USE  Skin/*PATHOLOGY  Support, U.S. Gov't, P.H.S.
       *Wuchereria bancrofti/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).