Document 0347
 DOCN  M9650347
 TI    A rapid and inexpensive method for processing induced sputum for
       detection of Pneumocystis carinii.
 DT    9605
 AU    Rocha P; Awe RJ; Guy ES; Gaines V; Banez EI; Stager CE; Guntupalli KK;
       Department of Pulmonary Medicine, Ben Taub General Hospital,; Houston,
       TX 77030, USA.
 SO    Am J Clin Pathol. 1996 Jan;105(1):52-7. Unique Identifier : AIDSLINE
       MED/96143465
 AB    The authors describe a method to process induced sputum specimens for
       detection of Pneumocystis carinii which is simple, rapid and
       inexpensive. Induced sputum and bronchoalveolar lavage (BAL) were
       obtained within a 24-hour period from 41 patients who were HIV-positive
       and had pulmonary symptoms suspicious for P carinii pneumonia. Induced
       sputum or BAL fluid was placed into Saccomanno's fixative, blended, and
       centrifuged. The sediment was stained for P carinii cysts by a modified
       method with Fungi-Fluor Solution A (Polysciences, Warington, PA) and the
       Genetic Systems Pneumocystis carinii Immunofluorescence Antibody
       (Genetic Systems, Seattle, WA). The Genetic Systems stain on the BAL
       specimen was positive in 35 patients and was the standard for
       comparison. With a single induced sputum, the Genetic Systems stain
       detected 31 (89%) positive patients, whereas the Fungi-Fluor stain
       detected 21 (60%). The sensitivity for detecting P carinii cysts in
       induced sputum was significantly greater (P < 0.05) for the Genetic
       Systems stain.
 DE    AIDS-Related Opportunistic Infections/*DIAGNOSIS  Bronchoalveolar Lavage
       Fluid/MICROBIOLOGY  Comparative Study  Fixatives  Fluorescent Antibody
       Technique, Direct  Human  Pneumocystis carinii/*ISOLATION & PURIF
       Pneumonia, Pneumocystis carinii/*DIAGNOSIS  Sensitivity and Specificity
       Specimen Handling/*METHODS  Sputum/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).