Document 0353
 DOCN  M9650353
 TI    Molecular analysis of human T-cell lymphotropic virus type II from Wayuu
       Indians of Colombia demonstrates two subtypes of HTLV-IIb.
 DT    9605
 AU    Switzer WM; Owen SM; Pieniazek DA; Nerurkar VR; Duenas-Barajas E;
       Heneine W; Lal RB; Retrovirus Diseases Branch, Centers for Disease
       Control and; Prevention, Atlanta, Georgia 30333, USA.
 SO    Virus Genes. 1995;10(2):153-62. Unique Identifier : AIDSLINE
       GENBANK/U12795
 AB    Studies of the genetic heterogeneity of human T-cell lymphotropic virus
       type II (HTLV-II) have revealed the presence of two genetic subtypes,
       termed HTLV-IIa and HTLV-IIb. The HTLV-IIb subtype encodes an
       immunodominant epitope present at the C-terminus of the extended Tax
       protein and, by using an LTR-based, restriction fragment-length
       polymorphism (RFLP) assay, can be further classified into IIb60-IIb5,
       with HTLV-IIb1 (Central Amerindian-like) and HTLV-IIb5 (North
       Amerindian-like) being characteristic subtypes for Native American
       Indians. To determine the antigenic and genetic heterogeneity among
       HTLV-II-infected South Amerindians, we used a Tax synthetic peptide
       immunoassay on serum, and RFLP and phylogenetic analysis on LTR
       sequences amplified from genomic DNA from four Wayuu Indians of
       Colombia. The Wayuu specimens displayed seroreactivity to the
       immunodominant epitope located in the extended Tax region, as predicted,
       and demonstrated genetic heterogeneity by the presence of both the IIB1
       (Wyu1, Zuc31) and IIb5 (Wyu2, Zuc42) subtypes sequences within separate
       phylogroups represented by the Guaymi Indian (IIb1) and North Amerindian
       (IIb5) sequences, respectively. Sequence analysis showed that major LTR
       regulatory motifs and the cis-acting repressive elements in the LTR RNA
       secondary structure were relatively conserved in both Wayuu subtypes,
       but the predicted secondary structure of the rex response stem loop in
       the Wyu2 (IIb5) LTR sequence was 45 nucleotides (nt) and 95 nt longer
       than that observed in the Wyu1 (IIb1) and G12.1 (IIb1) LTR sequences,
       respectively. These results extend our knowledge of the genetic
       heterogeneity of HTLV-II in South Amerindians.
 DE    Amino Acid Sequence  Antigens, Viral/IMMUNOLOGY  Base Sequence  Cell
       Line, Transformed  Colombia/EPIDEMIOLOGY  DNA, Viral/CHEMISTRY/GENETICS
       Gene Products, tax/GENETICS  Genes, Viral  Genetic Heterogeneity  Human
       HTLV-II/*CLASSIFICATION/GENETICS/IMMUNOLOGY/ISOLATION & PURIF  HTLV-II
       Infections/EPIDEMIOLOGY/VIROLOGY  *Indians, South American  Molecular
       Sequence Data  Nucleic Acid Conformation  Phylogeny  Repetitive
       Sequences, Nucleic Acid  Serotyping  JOURNAL ARTICLE

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