Document 0378
 DOCN  M9650378
 TI    HLA-B27-restricted cytotoxic T lymphocyte responses to arthritogenic
       enterobacteria or self-antigens are dominated by closely related TCRBV
       gene segments. A study in patients with reactive arthritis.
 DT    9605
 AU    Duchmann R; May E; Ackermann B; Goergen B; Meyer zum Buschenfelde KH;
       Marker-Hermann E; First Department of Medicine, Johannes Gutenberg
       University of; Mainz, Germany.
 SO    Scand J Immunol. 1996 Jan;43(1):101-8. Unique Identifier : AIDSLINE +
 AB    Identification of the T-cell receptors (TCR) used by synovial cytotoxic
       T lymphocytes (CTL) of patients with reactive arthritis (ReA) may be
       crucial to better understanding the pathogenetic mechanism underlying
       the HLA-B27 association of spondylarthropathies. The authors, therefore,
       sequenced 25 TCRB chains from HLA-B27-restricted CD8+ CTL clones and two
       clonal lines specific for self- or Yersinia enterocolitica antigen
       isolated from synovial fluids of 3 HLA-B27+ patients with ReA and PBL of
       one healthy HLA-B27+ individual. Fourteen non-HLA-B27-restricted CTL
       served as controls. Both autoreactive and Y. enterocolitica specific
       HLA-B27-restricted CTL used a highly limited set of VB genes with
       preferential rearrangement of three closely related VB families (VB 13,
       14, 17), suggesting that these families contain a preferred site for
       contact with the HLA-B27 molecule. In addition, the presence of limited
       TCRBJ usage, limited heterogeneity in CDR3 sequences and dominant clones
       from individual donors among these CTL indicate that TCRB chain usage is
       further restricted by a limited set of peptides bound to the HLA-B27
       molecule. Limited TCR usage by SF CTL of ReA patients may lay a basis
       for therapeutical manipulation of the T-cell response in the
       spondylarthropathies.
 DE    Adult  Amino Acid Sequence  Antigens, Bacterial/IMMUNOLOGY  Arthritis,
       Reactive/*IMMUNOLOGY  Autoantigens/*IMMUNOLOGY  Chlamydia
       trachomatis/IMMUNOLOGY  Chlamydia Infections/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  DNA/ANALYSIS  Gene Rearrangement, beta-Chain
       T-Cell Antigen Receptor/GENETICS  Human  HLA-B27 Antigen/*IMMUNOLOGY
       Male  Molecular Sequence Data  Polymerase Chain Reaction  Receptors,
       Antigen, T-Cell, alpha-beta/*GENETICS  Salmonella typhimurium/IMMUNOLOGY
       Salmonella Infections/IMMUNOLOGY  Support, Non-U.S. Gov't  Synovial
       Fluid/IMMUNOLOGY/MICROBIOLOGY  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       Yersinia enterocolitica/*IMMUNOLOGY/ISOLATION & PURIF  Yersinia
       Infections/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).