Document 0422
 DOCN  M9650422
 TI    Comparative study of the cell tropism of feline immunodeficiency virus
       isolates of subtypes A, B and D classified on the basis of the env gene
       V3-V5 sequence.
 DT    9605
 AU    Hohdatsu T; Hirabayashi H; Motokawa K; Koyama H; Department of
       Veterinary Infectious Diseases, School of; Veterinary Medicine and
       Animal Sciences, Kitasato University,; Aomori, Japan.
 SO    J Gen Virol. 1996 Jan;77 ( Pt 1):93-100. Unique Identifier : AIDSLINE
       MED/96145138
 AB    Feline immunodeficiency virus (FIV) isolates have been classified into
       subtypes A, B, C and D based on the env gene V3-V5 sequence. The cell
       tropism of seven new Japanese isolates and a Petaluma (prototype)
       isolate of FIV, which classified into subtypes A, B and D, for feline
       lymphoblastoid and feline fibroblastoid cell lines was compared. FeT-1
       (CD4+/-, CD8-, AND CD9+2) and Kumi-1 (CD4+2, CD8- and CD9+2) cells were
       used as the interleukin-2 (IL-2)-dependent feline T-lymphocyte cell
       lines and FeT-J (CD4+, CD8+/- and CD9+2) and 3201 (CD4+2, CD8+ and CD9-)
       cells were used as the IL-2- independent feline T-lymphocyte cell lines.
       The feline fibroblastoid cell lines used were Crandell feline kidney
       (CrFK) and fewf-4 (both CD4-, CD8- and CD9+2) cells. All FIV isolates
       replicated in all lymphoblastoid cell lines used. All isolates showed
       the greatest cytopathogenicity for Kumi-1 cells. All isolates replicated
       even in the CD9-negative 3201 cells. More isolates caused persistent
       infection in IL-2-independent cell lines than in IL-2-dependent cell
       lines. The number of subtype B isolates that established persistent
       infection was limited, only one of four strains. Only the subtype A
       isolates replicated in CrFK cells, whereas none of the isolates
       replicated in fewf-4 cells, which have similar cell surface markers to
       CrFK cells. The subtype A viruses (CrFK/Petaluma, CrFK/Sendai-1) growing
       in CrFK cells showed greater cytopathogenicity for lymphoblastoid cell
       lines than did those (FL-4/Petaluma, Kumi-1/Sendai-1) growing in a
       lymphoblastoid cell line.
 DE    Animal  Base Sequence  Cats  Cell Line  Comparative Study
       Cytopathogenic Effect, Viral  Fibroblasts/VIROLOGY  Flow Cytometry
       *Genes, env  Genes, gag  Human  Immunodeficiency Virus,
       Feline/CLASSIFICATION/GENETICS/  *PHYSIOLOGY  Immunophenotyping
       Lymphoid Tissue/CYTOLOGY/VIROLOGY  Molecular Sequence Data  Support,
       Non-U.S. Gov't  *Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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