Document 0425
 DOCN  M9650425
 TI    The role of BALB/c donor CD8+ lymphocytes in graft-versus-host disease
       in (BALB/c x A/J)F1 (CAF1) mice.
 DT    9605
 AU    Vidal S; Labrador M; Rodriguez-Sanchez JL; Gelpi C; Department of
       Immunology, Hospital de Sant Pau, Universitat; Autonoma de Barcelona,
       Avgda, Spain.
 SO    J Immunol. 1996 Feb 1;156(3):997-1005. Unique Identifier : AIDSLINE
       MED/96144319
 AB    To investigate the role of donor T lymphocyte subsets in the development
       of chronic graft-vs-host disease (GVHD) induced in (BALB/c x A/J)F1
       (CAF1) mice by injecting BALB/c lymphoid cells, we analyzed the effect
       that CD8+ cell removal from donor inoculum has on the manifestation of
       the disease. Compared with age- and sex-matched CAF1 mice injected with
       whole lymphocyte inoculum, CAF1 mice injected with CD8(+)-depleted
       inoculum exhibited: 1) a higher incidence and exacerbation of nephritis
       by immunocomplexes; 2) higher (five- to sevenfold) spontaneous IL-4
       production; 3) higher frequency titer and precocity of anti-dsDNA,
       anti-histone, and IgM and IgG rheumatoid factors; 4) a dramatic change
       in the frequency and titer of anti-U1 small nuclear ribonucleoprotein
       Abs; and 5) a markedly decreased engraftment (10- to 15-fold) on BALB/c
       donor lymphocytes. In contrast, rheumatoid arthritis-like disease, a
       later clinical manifestation of the GVHD in CAF1 + BALB/c model, is not
       present in the CD8(+)-depleted model (CAF1 + CD8-BALB/c). Considered
       together, these data suggest that CD8+ donor T lymphocytes play an
       important role in the degree of chimerism, modulation of the response to
       autoantigens, and clinical aspects developed in the GVHD model presented
       here.
 DE    Animal  Cell Division/IMMUNOLOGY  Chimera/IMMUNOLOGY  *Crosses, Genetic
       CD8-Positive T-Lymphocytes/IMMUNOLOGY/*TRANSPLANTATION  Female  Graft vs
       Host Disease/*IMMUNOLOGY  Immunophenotyping  Interleukin-2/BIOSYNTHESIS
       Interleukin-4/BIOSYNTHESIS  Lymphocyte Transformation/GENETICS  Mice
       Mice, Inbred A  Mice, Inbred BALB C  Spleen/CYTOLOGY  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).