Document 0430
 DOCN  M9650430
 TI    Abnormal thymocyte subset distribution and differential reduction of
       CD4+ and CD8+ T cell subsets during peripheral maturation in
       diabetes-prone BioBreeding rats.
 DT    9605
 AU    Groen H; Klatter FA; Brons NH; Mesander G; Nieuwenhuis P; Kampinga J;
       Department of Histology and Cell Biology, University of; Groningen, The
       Netherlands.
 SO    J Immunol. 1996 Feb 1;156(3):1269-75. Unique Identifier : AIDSLINE
       MED/96144354
 AB    In this study we quantified CD8+ and CD4+ T cells in T lymphocytopenic
       BB rats as compared with control rats at given stages along the
       maturational pathway from immature thymocytes to mature peripheral T
       cells. Our results show that BB rats exhibit abnormal thymocyte subset
       distribution. Numbers of mature TCRhigh/CD4-8+ thymocytes, and also
       their TCRhigh/CD4+8+ precursors were decreased, as were levels of CD8
       expression on all thymocyte subsets investigated. By analogy with mouse
       thymocyte development, these findings suggest a decreased efficiency for
       positive selection of CD8 precursors in BB rats. Furthermore, as related
       to the number of available mature TCRhigh single positive thymocytes,
       numbers of CD4+ and CD8+ T cells most recently migrated from the thymus
       were severely decreased in BB blood, indicating either reduced thymic
       output or rapid cell death after migration. Subsequently, in peripheral
       blood and cervical lymph nodes, a 95% decrease of CD8+ and a 50 to 80%
       decrease of CD4+ T cells were demonstrated upon maturation from recent
       thymic migrants to mature peripheral T cells, leaving the BB rat with a
       severely reduced T cell population, consisting of CD4+ T cells and a
       minute population of CD8+ T cells. The vast majority of the latter was
       found to have an immature peripheral phenotype. Possible consequences of
       our findings for the generation of autoreactive CD8+ T cells are
       discussed.
 DE    Age Factors  Animal  Antigens, CD8/BIOSYNTHESIS  Antigens,
       Thy-1/BIOSYNTHESIS  Cell Differentiation/IMMUNOLOGY  Cell
       Movement/IMMUNOLOGY  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Diabetes Mellitus,
       Insulin-Dependent/*IMMUNOLOGY  Lymph Nodes/CYTOLOGY  Lymphocyte Count
       Lymphopenia/*IMMUNOLOGY/PATHOLOGY  Male  Rats  Rats, Inbred BB  Rats,
       Inbred Strains  Receptors, Antigen, T-Cell/BIOSYNTHESIS  Support,
       Non-U.S. Gov't  Thymus Gland/*CYTOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).