Document 0432
 DOCN  M9650432
 TI    Limited T cell receptor usage by HTLV-I tax-specific, HLA class I
       restricted cytotoxic T lymphocytes from patients with HTLV-I associated
       neurological disease.
 DT    9605
 AU    Elovaara I; Utz U; Smith S; Jacobson S; Department of Neurology, Tampere
       University Hospital, Finland.
 SO    J Neuroimmunol. 1995 Dec;63(1):47-53. Unique Identifier : AIDSLINE
       MED/96110770
 AB    T cell receptor (TCR) V alpha and V beta chain usage of HTLV-I
       tax-specific, HLA class I restricted CD8+ cytotoxic T cells (CTL) was
       determined from lymphocytes obtained from peripheral blood of patients
       with HTLV-I associated neurological disease. To characterize TCR
       repertoire, CD8+ lymphocytes from peripheral blood were cloned in
       limiting dilution, and the resulting wells were screened for
       HTLV-I-specific precursor CTL activity. RNA was isolated from HLA-A2
       restricted HTLV-I tax peptide-specific (tax 11-19; LLFGYPVYV) CD8+ CTL
       lines and cDNA was analyzed by PCR amplification using V alpha and V
       beta chain family-specific oligonucleotide primers. The results indicate
       that CD8+ cytotoxic T cell lines from HLA-A2 HAM/TSP patients express a
       limited repertoire of T cell receptor chains which may correlate with
       duration and severity of disease. The restricted use of TCR genes
       expressed by antigen-specific CTL may play a critical role in the
       pathogenesis of HAM/TSP and may be of value in developing
       immunotherapeutic strategies that focus on eliminating these cells or
       inhibiting their activity.
 DE    Amino Acid Sequence  Base Sequence  Female  Gene Products,
       tax/*IMMUNOLOGY  Histocompatibility Antigens Class I/*IMMUNOLOGY  Human
       HTLV-I/*IMMUNOLOGY  Molecular Sequence Data  Paraparesis, Tropical
       Spastic/*IMMUNOLOGY  Peptide Fragments/IMMUNOLOGY  Receptors, Antigen,
       T-Cell, alpha-beta/*ANALYSIS  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       JOURNAL ARTICLE

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