Document 0452
 DOCN  M9650452
 TI    Complete development of Cryptosporidium parvum in bovine fallopian tube
       epithelial cells.
 DT    9605
 AU    Yang S; Healey MC; Du C; Zhang J; Department of Animal, Dairy and
       Veterinary Sciences, Utah State; University, Logan 84322-5600, USA.
 SO    Infect Immun. 1996 Jan;64(1):349-54. Unique Identifier : AIDSLINE
       MED/96110955
 AB    Cryptosporidium parvum is a coccidian parasite responsible for causing
       protracted and life-threatening diarrheal illness in immunocompromised
       humans, especially patients with AIDS. The lack of medications effective
       in treating people suffering from cryptosporidiosis has prompted the
       development of in vivo and in vitro models for this disease. This study
       is the first to demonstrate that C. parvum can complete its entire life
       cycle (from sporozoite to infective oocyst) in a primary culture of
       bovine fallopian tube epithelial (BFTE) cells. Scanning and transmission
       electron photomicrographs were used to detail the ultrastructure of
       individual parasitic stages. Successful infections were produced by
       inoculating cell cultures with either oocysts or purified sporozoites.
       Infection of BFTE cells with C. parvum close paralleled in vivo
       infections with regard to host cell location and chronology of parasite
       development. Infecting BFTE cells with sporulated oocysts provided a
       reproducible and quantitative cultivation system with significantly (P <
       or = 0.001) higher infection rates than in Madin-Darby canine kidney
       cells. Oocysts produced in BFTE cells were infective for
       immunosuppressed adult C57BL/6N mice. Cultivation of C. parvum in BFTE
       cells will facilitate the study of interactions between parasites and
       host cells as well as provide a reliable system for evaluating
       anticryptosporidial compound efficacy.
 DE    Animal  Cattle  Cells, Cultured  Child, Preschool  Comparative Study
       Cryptosporidium parvum/*GROWTH & DEVELOPMENT/ULTRASTRUCTURE
       Epithelium/PARASITOLOGY  Fallopian Tubes/CYTOLOGY/*PARASITOLOGY  Female
       Human  Kidney/CYTOLOGY/PARASITOLOGY  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).