Document 0457
 DOCN  M9650457
 TI    NF-kappa B-mediated chromatin reconfiguration and transcriptional
       activation of the HIV-1 enhancer in vitro.
 DT    9605
 AU    Pazin MJ; Sheridan PL; Cannon K; Cao Z; Keck JG; Kadonaga JT; Jones KA;
       Department of Biology, University of California, San Diego; (UCSD), La
       Jolla 92093-0347, USA.
 SO    Genes Dev. 1996 Jan 1;10(1):37-49. Unique Identifier : AIDSLINE
       MED/96136707
 AB    NF-kappa B is a potent inducible transcription factor that regulates
       many genes in activated T cells. In this report we examined the ability
       of different subunits of NF-kappa B to enhance HIV-1 transcription in
       vitro with chromatin templates. We find that the p65 subunit of NF-kappa
       B is a strong transcriptional activator of nucleosome-assembled HIV-1
       DNA, whereas p50 does not activate transcription, and that p65 activates
       transcription synergistically with Sp1 and distal HIV-1 enhancer-binding
       factors (LEF-1, Ets-1, and TFE-3). These effects were observed with
       chromatin, but not with nonchromatin templates. Furthermore, binding of
       either p50 or p65 with Sp1 induces rearrangement of the chromatin to a
       structure that resembles the one reported previously for integrated
       HIV-1 proviral DNA in vivo. These results suggest that p50 and Sp1
       contribute to the establishment of the nucleosomal arrangement of the
       uninduced provirus in resting T cells, and that p65 activates
       transcription by recruitment of the RNA polymerase II transcriptional
       machinery to the chromatin-repressed basal promoter.
 DE    Base Sequence  Binding Sites  Chromatin/CHEMISTRY/*GENETICS/METABOLISM
       Comparative Study  DNA-Binding Proteins/GENETICS  *Gene Expression
       Regulation, Viral  Hela Cells  Human  HIV Enhancer/*GENETICS  HIV Long
       Terminal Repeat  Molecular Sequence Data  Nucleosomes/GENETICS  NF-kappa
       B/CHEMISTRY/*GENETICS/ISOLATION & PURIF/METABOLISM  Repetitive
       Sequences, Nucleic Acid  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       Non-P.H.S.  Support, U.S. Gov't, P.H.S.  Trans-Activators  Transcription
       Factor, Sp1/GENETICS/METABOLISM  Transcription
       Factors/GENETICS/METABOLISM  *Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).