Document 0458
 DOCN  M9650458
 TI    Inactivation of HIV-1 by chemical disinfectants: sodium hypochlorite.
 DT    9605
 AU    Van Bueren J; Simpson RA; Salman H; Farrelly HD; Cookson BD; Laboratory
       of Hospital Infection, Central Public Health; Laboratory, London, UK.
 SO    Epidemiol Infect. 1995 Dec;115(3):567-79. Unique Identifier : AIDSLINE
       MED/96150221
 AB    The efficacy of sodium hypochlorite was assessed against human
       immunodeficiency virus type 1 suspended in low (8% v/v) or high (80%
       v/v) concentrations of serum or in a high (80%) concentration of blood.
       In the presence of 8% serum, 100 p.p.m. available chlorine in the
       disinfectant test mixture inactivated 3.75 log TCID50 HIV/ml within 30
       s. When the test mixture contained 80% serum, 500 p.p.m. available
       chlorine inactivated more than 4 log TCID50 HIV/ml in 1-2 min. Lower
       concentrations of available chlorine were unable to inactivate the virus
       completely. In the presence of 80% blood, 1000 p.p.m. available chlorine
       in the disinfectant test mixture was unable to inactivate 3.75 log
       TCID50 HIV/ml, although 2500 p.p.m. available chlorine was able to
       inactivate at least 1.5 log TCID50 HIV/ml. In all test mixtures, the
       chlorine rapidly became combined and thus less active. Our results
       emphasise the importance of cleaning prior to disinfection with sodium
       hypochlorite since it may prove to be ineffective in the presence of
       high levels of organic matter. In cases where prior cleaning is
       impossible, care must be taken to use the higher recommended
       concentration (a minimum of 10,000 p.p.m. available chlorine).
 DE    Cell Survival/DRUG EFFECTS  Cytopathogenic Effect, Viral/DRUG EFFECTS
       Disinfectants/*PHARMACOLOGY  Disinfection/METHODS  Human  HIV-1/*DRUG
       EFFECTS  Leukocytes, Mononuclear/DRUG EFFECTS/VIROLOGY  Sodium
       Hypochlorite/*PHARMACOLOGY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).