Document 0461
 DOCN  M9650461
 TI    CD43 and CD5 antibodies define four normal and neoplastic B-cell
       subsets: a three-color flow cytometric study.
 DT    9605
 AU    Lynch EF; Jones PA; Swerdlow SH; Department of Pathology, University of
       Pittsburgh School of; Medicine, Pennsylvania, USA.
 SO    Cytometry. 1995 Sep 15;22(3):223-31. Unique Identifier : AIDSLINE
       MED/96106606
 AB    CD43 antibodies are best known for identifying normal and neoplastic T
       cells and a subset of neoplastic B cells in paraffin sections. The
       frequency of nonneoplastic CD43 + B cells in different reactive
       settings, the proportion of B-cell neoplasms with small CD43+
       populations, and the relationship of CD43+ B cells was therefore studied
       in 138 specimens using three-color cytometric analysis. CD43 and CD5
       defined four normal B-cell subsets (CD43 + CD5 +, CD43 + CD5-, CD43 -
       CD5+, and CD43-CD5-). A significantly greater proportion of CD43+ B
       cells was found in cord blood and putative HIV+ blood samples than in
       normal control bloods. B-cell neoplasms derived from each of these four
       B-cell subsets were identified, with CD43+ /CD5+ and CD43-/CD5-
       neoplasms being most common. The predominant B-cell population
       coexpressed CD43 alone in 2/39 neoplasms and CD5 alone in four. A
       minority of cases showed heterogeneous CD43 expression. The B cells in
       two of three posttransplant lymphoproliferative disorders coexpressed
       CD43. B cells showed weaker CD43 staining than did T-cells (relative
       fluorescence 0.38 +/- 0.29). These findings support the concept that
       CD43 expression by neoplastic B cells is not an aberrant finding. CD43
       expression on normal and neoplastic B cells is independent of CD5
       expression even though CD43 and CD5 are frequently coexpressed. CD43
       expression by B cells sometimes might be underestimated in paraffin
       sections because it is much weaker than on T cells.(ABSTRACT TRUNCATED
       AT 250 WORDS)
 DE    Adult  Ammonium Chloride  Animal  Antibodies/*IMMUNOLOGY  Antigens,
       CD19/IMMUNOLOGY  Antigens, CD5/*IMMUNOLOGY  B-Lymphocyte
       Subsets/*IMMUNOLOGY  *Flow Cytometry  Human  Lymphoma,
       B-Cell/*IMMUNOLOGY  Reagent Kits, Diagnostic
       Sialoglycoproteins/*IMMUNOLOGY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).