Document 0468
 DOCN  M9650468
 TI    Major antigenic region on the integrase (IN) protein of human
       immunodeficiency virus type 1 determined by reactivity of human sera and
       a monoclonal antibody to IN protein.
 DT    9605
 AU    Tachibana Y; Yasuda A; Kurata T; Oka S; Shimada K; Kojima A; Department
       of Pathology, National Institute of Health, Tokyo,; Japan.
 SO    Clin Diagn Lab Immunol. 1994 Nov;1(6):678-83. Unique Identifier :
       AIDSLINE MED/96050890
 AB    The gene encoding the integrase (IN) protein of human immunodeficiency
       virus type 1 (HIV-1) was expressed in vaccinia virus and Escherichia
       coli, and sera from 55 HIV-1-infected individuals were examined for
       immunoreactivity to the recombinant IN proteins by Western immunoblot.
       Approximately 98% (54 of 55) of the HIV-1-infected individuals showed
       reactivity to both the full-length IN protein of 32 kDa (IN32 protein)
       and the carboxy-terminal portion of the IN protein (IN17 protein). Serum
       samples from only 6 of the 54 antibody-positive individuals and a
       monoclonal antibody against the IN protein, 6F4, reacted with the
       amino-terminal portion of the IN protein (IN15 protein). The eight AIDS
       patients tested were seronegative to IN15 protein. The magnitude of
       reactivity to the recombinant IN proteins decreased slightly in the
       progression of the course of HIV-1 infection. These results suggest that
       a B-cell immunodominant epitope(s) on the IN protein is located on the
       C-terminal IN17 portion and that a minor epitope(s) recognizable by 6F4
       and by rare patients is on the N-terminal IN15 portion.
 DE    Amino Acid Sequence  Antibodies, Monoclonal/IMMUNOLOGY  Antibody
       Specificity  Base Sequence  Blotting, Western  DNA
       Nucleotidyltransferases/CHEMISTRY/GENETICS/*IMMUNOLOGY
       Epitopes/IMMUNOLOGY  Human  HIV Antigens/BLOOD/IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Molecular Sequence Data  Recombinant
       Proteins/IMMUNOLOGY  Virus Integration/GENETICS/*IMMUNOLOGY  JOURNAL
       ARTICLE

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