Document 0470
 DOCN  M9650470
 TI    Characterization of circulating CD4+ CD8+ lymphocytes in healthy
       individuals prompted by identification of a blood donor with a markedly
       elevated level of CD4+ CD8+ lymphocytes.
 DT    9605
 AU    Prince HE; Golding J; York J; Cellular Immunology Laboratory, American
       Red Cross Blood and; Tissue Services, Southern California Region, Los
       Angeles, USA.
 SO    Clin Diagn Lab Immunol. 1994 Sep;1(5):597-605. Unique Identifier :
       AIDSLINE MED/96050877
 AB    During flow cytometric analysis of lymphocytes from healthy donors, we
       identified a donor (donor A) with 22% CD4+ CD8+ cells (versus values of
       < 4% for 65 other controls). To determine if CD4+ CD8+ cells from donor
       A and other controls were similar, we first defined the phenotypic
       profile of control CD4+ CD8+ cells. Enriched CD4+ CD8+ cell populations
       for 10 controls were prepared by a two-step positive selection scheme
       with anti-CD4-coated magnetic beads and anti-CD8-coated culture flasks;
       the selected population averaged 69% CD4+ CD8+ cells and 31% CD4+ CD8-
       cells. For all 10 controls, two subsets of CD4+ CD8+ cells, CD4dim
       CD8bright and CD4bright CD8dim, were observed. Phenotypic profiles of
       these two CD4+ CD8+ subsets were defined by pairing anti-CD8 with other
       monoclonal antibodies, and the profiles were compared with each other
       and with those of CD4+ CD8-, CD4- CD8bright, and CD4- CD8dim cells.
       CD8bright and CD4bright CD8dim cells differed in their proportions of
       CD62-L+ cells and in their levels of CD11a and CD2 expression. Both CD4+
       CD8+ subsets resembled CD4+ CD8- cells in CD45RA, CD45RO, and CD25
       expression; the comparable CD- CD8+ cells in CD62-L expression; and CD4-
       CD8bright cells in CD11b, CD11b, CD16/56, and CD28 expression. CD38
       expression in both CD4+ CD8+ subsets was decreased compared with those
       of other cell subsets. Whereas control CD4+ CD8+ cells averaged 33%
       CD4dim CD8bright, CD4+ CD8+ cells from donor A were > 90% CD4dim
       CD8bright. Donor A CD4dim CD8bright cells exhibited proportional
       decreases in CD25 and CD62-L expression and increases in CD11b and CD54
       expression compared with those of control CD4dim CD8bright
       cells.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    *Blood Donors  Case Report  CD4-CD8 Ratio  CD4-Positive
       T-Lymphocytes/*CYTOLOGY/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*CYTOLOGY/IMMUNOLOGY  Female  Flow Cytometry  Human
       Immunophenotyping  Lymphocyte Count  Lymphocyte Subsets/IMMUNOLOGY
       Lymphocyte Transformation/IMMUNOLOGY  Male  Middle Age  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).