Document 0471
 DOCN  M9650471
 TI    Interleukin-2-inducible natural immune (lymphokine-activated killer
       cell) responses as a functional correlate of progression to AIDS.
 DT    9605
 AU    Brenner BG; Gornitsky M; Wainberg MA; McGill AIDS Centre, Lady Davis
       Institute--Jewish General; Hospital, Montreal, Quebec, Canada.
 SO    Clin Diagn Lab Immunol. 1994 Sep;1(5):538-44. Unique Identifier :
       AIDSLINE MED/96050868
 AB    The functions of natural killer (NK) cells and their
       interleukin-2-deducible counterparts, lymphokine-activated killer (LAK)
       cells, are often impaired in human immunodeficiency virus (HIV)-infected
       individuals. A statistical approach was used to establish if changes in
       LAK activity were associated with antiviral drug therapy, HIV-1 burden,
       or lymphocyte subset alterations. Our study group included 61
       HIV-positive subjects without any opportunistic infections (OI-), 16 of
       whom received zidovudine (AZT), and 97 HIV-positive individuals with
       AIDS-related infection (OI+), 50 of whom received AZT. As expected,
       there was a stepwise decrease in total lymphocyte numbers in OI+ groups
       as a result of the selective loss of CD4+ cells. The groups receiving
       AZT therapy had fewer CD4+ cells but lower circulating p24 antigen
       levels than corresponding untreated groups did. No significant changes
       in the relative proportions or absolute numbers of CD56+ subsets in
       HIV-positive groups could be ascribed to OI status or AZT intervention.
       LAK cell cytotoxic responses, measured as LU20 values (which give a
       measure of 20% cytolysis of target cells), lysis per unit CD56+ NK cell,
       or lysis per unit blood volume, declined in OI+ groups. No main or
       interactive effects of AZT therapy on LAK activities were observed.
       Multivariate general linear models were used to determine the
       interactive effects of NK- and T-cell subsets on measured LAK cell
       numbers were added negative and positive predictors of LAK activity,
       respectively. These findings indicate that declines in NK-mediated LAK
       cell responses serve as functional correlates of progression in
       HIV-infected individuals.
 DE    Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Antigens, CD56/ANALYSIS
       AIDS-Related Opportunistic Infections/IMMUNOLOGY  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  Human  Immunity, Natural/IMMUNOLOGY
       Interleukin-2/*PHARMACOLOGY  Killer Cells, Lymphokine-Activated/*DRUG
       EFFECTS/*IMMUNOLOGY  Linear Models  Lymphocyte Count  Multivariate
       Analysis  Support, Non-U.S. Gov't  T-Lymphocyte
       Subsets/CYTOLOGY/IMMUNOLOGY  Zidovudine/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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