Document 0472
 DOCN  M9650472
 TI    Blood monocytes from most human immunodeficiency virus type 1-infected
       patients do not carry proviral DNA.
 DT    9605
 AU    Shen Y; Rudnik J; Cassol S; Drouin J; Cameron W; Izaguirre CA; Filion
       LG; Department of Microbiology, University of Ottawa General; Hospital,
       Ontario, Canada.
 SO    Clin Diagn Lab Immunol. 1994 Sep;1(5):531-7. Unique Identifier :
       AIDSLINE MED/96050867
 AB    In blood, the CD4+ T cells of patients with human immunodeficiency virus
       type 1 (HIV-1) harbor HIV-1; however, whether the CD4+ blood monocytes
       carry the virus is controversial. Tissue macrophages are known to be
       infected. To determine in blood monocytes from HIV-1-seropositive
       patients contain HIV-1, we separated monocytes and T-cell subsets by
       using monoclonal antibodies bound to magnetic beads and by monocyte
       adherence to glass. Monocytes were cultured with macrophage
       colony-stimulating factor, granulocyte-macrophage colony-stimulating
       factor, and interleukin-3. After 14 days in culture, cells were analyzed
       for the presence of HIV-1 antigen and multinucleated giant cells (MGCs).
       Freshly isolated cell subsets were analyzed for HIV-1 proviral DNA by
       PCR with modified env (SK68i and SK69i2) and gag (SK145i and SK150)
       primers. We found that (i) monocytes cultured without depletion of CD4+
       T cells (11 of 11 patients) were HIV-1 antigen positive and showed
       dramatically increased spontaneous formation of MGCs (ii) monocytes
       cultured after depletion of CD4+ T cells (three experiments) were HIV-1
       antigen negative and showed markedly decreased MGC formation, and (iii)
       in specimens from 14 patients subsequently analyzed by PCR, purified
       CD4+ T cells were positive for HIV-1 proviral DNA in all patients. In 11
       of 14 patients (79%), the monocyte fractions were HIV-1 proviral DNA
       negative, while in the remaining 3 patients, the monocytes were positive
       for HIV-1 proviral DNA. In conclusion, the major reservoir for HIV-1
       infection in human peripheral blood is the CD4+ T cell (14 of 14
       cases).(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Acquired Immunodeficiency Syndrome/*BLOOD  Antigens, Viral/IMMUNOLOGY
       Base Sequence  Cells, Cultured/IMMUNOLOGY/VIROLOGY  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY/VIROLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY/VIROLOGY  DNA, Viral/ANALYSIS  Human
       HIV-1/GENETICS/*IMMUNOLOGY  Magnetics  Microspheres  Molecular Sequence
       Data  Monocytes/IMMUNOLOGY/*VIROLOGY  Polymerase Chain Reaction
       Support, Non-U.S. Gov't  T-Lymphocyte Subsets/IMMUNOLOGY/VIROLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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